Fu Yanjie, Zhang Jinghua, Bai Hansheng, Chen Yuanzheng, Liu Rongpeng, Bai Nan
Department of Surgery, Linyi People's Hospital, Linyi City, Shandong Province 276000, China.
Heze Medical College, Heze City, Shandong Province 274000, China.
Hum Immunol. 2018 Nov;79(11):817-820. doi: 10.1016/j.humimm.2018.07.002. Epub 2018 Jul 6.
Sepsis is a clinical syndrome that is frequently observed after injury or infection, representing a leading cause of mortality worldwide. CD86 (B7-2) is a co-stimulatory molecule on antigen-presenting cells, and plays critical roles in immune responses.
A total of 135 sepsis patients and 151 healthy controls were recruited in the current case-control study. Hardy-Weinberg equilibrium (HWE) conformity was examined to assess the representativeness of the study population. CD86 gene polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The relative expression of CD86 mRNA was estimated via quantitative real-time PCR (qRT-PCR). Chi-square test was performed to estimate the associations between CD86 gene polymorphisms and sepsis risk, and the results were presented through odds ratio (OR) and 95% confidence intervals (CI).
The genotype distributions of CD86 polymorphisms in the case and control groups conformed to HWE. The GA genotype of the polymorphism rs1129055 was significantly correlated with an increased risk of sepsis (OR = 2.540, 95%CI = 1.288-5.008). The TT genotype of rs1915087 was a risk factor for sepsis (OR = 2.769, 95%CI = 1.292-5.935). High linkage disequilibrium was observed between the two polymorphisms (D' = 1.0, r = 0.955). However, no significant association was observed between CD86 polymorphisms and its gene expressions (P > 0.05 for all).
CD86 gene polymorphisms rs1129055 and rs1915087 may increase the risk of sepsis.
脓毒症是一种常在受伤或感染后出现的临床综合征，是全球范围内主要的死亡原因之一。CD86（B7-2）是抗原呈递细胞上的一种共刺激分子，在免疫反应中起关键作用。
在当前这项病例对照研究中，共招募了135例脓毒症患者和151名健康对照者。检验哈迪-温伯格平衡（HWE）符合度以评估研究人群的代表性。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对CD86基因多态性进行基因分型。通过定量实时PCR（qRT-PCR）估计CD86 mRNA的相对表达。进行卡方检验以评估CD86基因多态性与脓毒症风险之间的关联，结果以比值比（OR）和95%置信区间（CI）表示。
病例组和对照组中CD86多态性的基因型分布符合HWE。多态性rs1129055的GA基因型与脓毒症风险增加显著相关（OR = 2.540，95%CI = 1.288 - 5.008）。rs1915087的TT基因型是脓毒症的一个风险因素（OR = 2.769，95%CI = （此处原文有误，应为1.292 - 5.935））。观察到这两个多态性之间存在高度连锁不平衡（D' = 1.0，r = 0.955）。然而，未观察到CD86多态性与其基因表达之间存在显著关联（所有P值均>0.05）。
CD86基因多态性rs1129055和rs1915087可能会增加脓毒症风险。
