用甲磺酸乙酯诱导家蚕雄蚕生殖细胞中的显性致死突变

Induction of dominant lethals with ethyl methane-sulfonate in male germ cells of mulberry silkwork, Bombyx mori l.

作者信息

Datta R K, Sengupta K, Das S K

出版信息

Mutat Res. 1978 Jan;56(3):299-304. doi: 10.1016/0027-5107(78)90197-5.

PMID:203849

Abstract

Sensitivity of male germ cells in the mulberry silkworm, Bombyx mori L., to ethyl methanesulfonate (EMS) was determined by treating newly emerged 5th- instar larvae, and 2-day- and 7-day-old pupae with 3 concentrations, 0.05, 0.1 and 0.15%, of the mutagen. The frequency of dominant-lethal mutations induced by EMS treatment was used as the parameter for the study. Spermatids and spermatozoa were markedly sensitive to EMS. Statistical analysis confirmed that differences in respect of percentage of egg hatch among the 3 different treatments as well as the interactions between the 3 factors, e.g. stages, hatchability and EMS treatment, were highly significant.

摘要

通过用0.05%、0.1%和0.15%这3种浓度的诱变剂甲磺酸乙酯（EMS）处理刚羽化的5龄幼虫、2日龄和7日龄蛹，测定了家蚕（Bombyx mori L.）雄性生殖细胞对EMS的敏感性。以EMS处理诱导的显性致死突变频率作为研究参数。精细胞和精子对EMS明显敏感。统计分析证实，3种不同处理之间的卵孵化率差异以及阶段、孵化率和EMS处理这3个因素之间的相互作用具有高度显著性。