Generoso W M
Basic Life Sci. 1980;15:411-20. doi: 10.1007/978-1-4684-3842-0_28.
The fertilized egg may influence the yield of dominant-lethal mutations produced from chemical treatment of male postmeiotic germ cells to a small or large extent depending upon the mutagen used and the competence of the egg to repair the premutational lesions induced. The strain of females has little influence on the yield of dominant-lethal mutations induced by triethylenemelamine or ethyl methane-sulfonate in spermatids and spermatozoa, but it has a large influence in the case of isopropyl methanesulfonate. In addition to this difference, triethylenemelamine and ethyl methanesulfonate induce high levels of heritable translocations at these germ cell stages whereas isopropyl methanesulfonate is practically ineffective, even though doses of these chemicals produced comparable levels of dominant-lethal mutations. These differences between ethyl methanesulfonate and triethylenemelamine on one hand and isopropyl methanesulfonate on the other were hypothesized to be a function of the types of chromosomal lesions present at the time of repair activity and whether or not chromosomal aberrations were already fixed at the time of postfertilization pronuclear DNA synthesis.
受精卵可能会在很大程度上影响化学处理雄性减数分裂后生殖细胞所产生的显性致死突变的产量，这取决于所使用的诱变剂以及卵子修复诱导产生的前突变损伤的能力。雌性品系对三乙烯三聚氰胺或甲磺酸乙酯在精子细胞和精子中诱导产生的显性致死突变的产量影响很小，但对甲磺酸异丙酯而言影响很大。除了这种差异之外，三乙烯三聚氰胺和甲磺酸乙酯在这些生殖细胞阶段会诱导产生高水平的可遗传易位，而甲磺酸异丙酯实际上没有效果，尽管这些化学物质的剂量产生了相当水平的显性致死突变。一方面甲磺酸乙酯和三乙烯三聚氰胺与另一方面甲磺酸异丙酯之间的这些差异被认为是修复活动时存在的染色体损伤类型以及受精后原核DNA合成时染色体畸变是否已经固定的函数。
