Environmental Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Delhi, India.
Hum Exp Toxicol. 2010 May;29(5):351-8. doi: 10.1177/0748233710363334.
A number of studies have focused attention on various biochemical abnormalities evoked due to exposure to organochlorine pesticides (OCPs). The aim of the present study was to analyze the OCP residues in maternal and cord blood of women and assess the levels of different non-enzymatic oxidative stress markers as well as to establish correlation with OCP levels, if any. Thirty women in each group of full-term delivery (FTD; > or =37 weeks of gestation) and preterm delivery (PTD; <37 weeks of gestation) were enrolled in this study. Levels of OCPs like Hexachlorocyclohexane (HCH), endosulfan, p,p(') Dichlorodiphenyldichloroethylene (DDE) and p,p' Dichlorodiphenyltrichloroethane (DDT) were analyzed by gas chromatography. Non-enzymatic oxidative stress was measured by the quantification of malondialhyde (MDA), protein carbonyl, reduced glutathione (GSH) and ferric-reducing ability of plasma (FRAP). MDA and protein carbonyl levels were increased significantly, while the levels of GSH and FRAP were decreased in PTD in comparison to FTD cases. We have observed higher levels of beta-HCH and alpha-endosulfan and increased oxidative stress in PTD than FTD cases. In PTD cases, a significant positive correlation was observed between maternal blood levels of beta-HCH and MDA (r = .78), beta-HCH and GSH (r = -.65), gamma-HCH and MDA (r = .89), gamma-HCH and GSH (r = -.74) and alpha-endosulfan and MDA (r = .54) in PTD cases. We also found significant correlations between cord blood levels of beta-HCH and MDA (r = .59), beta-HCH and GSH (r = -.69), gamma-HCH and MDA (r = .62) and alpha-endosulfan and MDA (r = .54) in PTD cases. In conclusion, our results suggest that higher levels of some of the OCP residues may be associated with PTD and increased oxidative stress.
一些研究已经关注了由于接触有机氯农药（OCPs）而引起的各种生化异常。本研究的目的是分析产妇和脐带血中的 OCP 残留，并评估不同非酶抗氧化应激标志物的水平，并确定与 OCP 水平的相关性（如果有）。本研究纳入了 30 名足月分娩（FTD；≥37 周妊娠）和早产（PTD；<37 周妊娠）的妇女。采用气相色谱法分析 OCP 如六氯环己烷（HCH）、硫丹、p,p(')二氯二苯二氯乙烯（DDE）和 p,p'二氯二苯三氯乙烷（DDT）的水平。通过定量测定丙二醛（MDA）、蛋白质羰基、还原型谷胱甘肽（GSH）和血浆铁还原能力（FRAP）来测量非酶抗氧化应激。与 FTD 病例相比，PTD 中 MDA 和蛋白质羰基水平显著升高，而 GSH 和 FRAP 水平降低。与 FTD 病例相比，我们观察到 PTD 中β-HCH 和α-硫丹水平较高，氧化应激增加。在 PTD 病例中，母体血液中β-HCH 与 MDA（r =.78）、β-HCH 与 GSH（r = -.65）、γ-HCH 与 MDA（r =.89）、γ-HCH 与 GSH（r = -.74）和α-硫丹与 MDA（r =.54）之间存在显著正相关。我们还发现脐带血中β-HCH 与 MDA（r =.59）、β-HCH 与 GSH（r = -.69）、γ-HCH 与 MDA（r =.62）和α-硫丹与 MDA（r =.54）之间存在显著相关性。总之，我们的结果表明，一些 OCP 残留水平较高可能与 PTD 和氧化应激增加有关。
