Ingels K J, Kortmann M J, Nijziel M R, Graamans K, Huizing E H
Dept. of O.R.L., University Hospital Utrecht, The Netherlands.
Rhinology. 1991 Mar;29(1):17-26.
Investigation of the effect of environmental and pharmacological factors on human respiratory epithelium requires standardization of measuring conditions. Ciliary beat frequency (CBF) and its shift were determined photo-electrically in 43 biopsies of human nasal mucosa. Curette biopsies were compared to forceps biopsies. CBF variation between three different cells of one biopsy sample did not differ for the two biopsy techniques. The ciliated cells of forceps biopsy specimens showed a more constant beating pattern which resulted in a small CBF shift. It appeared that in studying ciliary activity a continuous layer of ciliated cells which is in contact with the basal membrane is required. Therefore forceps biopsies are preferable to curette biopsies. The environmental temperature has to remain constant since CBF is temperature dependent. The pH and osmolarity of the medium do not influence CBF when kept within a certain range. No effect of medium superfusion flow rate was seen.
研究环境和药理学因素对人呼吸道上皮的影响需要测量条件的标准化。通过光电方法测定了43份人鼻黏膜活检样本的纤毛摆动频率（CBF）及其变化。将刮匙活检与钳取活检进行了比较。两种活检技术在一个活检样本的三种不同细胞之间的CBF变化并无差异。钳取活检标本的纤毛细胞显示出更稳定的摆动模式，导致CBF变化较小。似乎在研究纤毛活动时，需要一层与基底膜接触的连续纤毛细胞层。因此，钳取活检优于刮匙活检。由于CBF受温度影响，环境温度必须保持恒定。当培养基的pH值和渗透压保持在一定范围内时，不会影响CBF。未观察到培养基灌注流速的影响。
