Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
PLoS One. 2010 Apr 6;5(4):e10032. doi: 10.1371/journal.pone.0010032.
Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis.
METHODOLOGY/PRINCIPAL FINDINGS: Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal imaging displayed similar temporal trends in cellular recruitment of phagocytes to the biomaterials compared to histological analysis.
CONCLUSIONS/SIGNIFICANCE: Histological analysis similarity validates this technique as a novel, rapid approach for screening biocompatibility of implanted materials. Through this technique there exists the possibility to rapidly screen large libraries of polymers in vivo.
由于生物相容性差，许多材料不适合医用。最近，生物材料高通量合成技术的进步显著增加了潜在生物材料的数量，然而当前的生物相容性分析方法耗时且需要组织学分析。
方法/主要发现：在这里，我们开发了一种快速、非侵入性的方法，用于体内定量分析植入生物材料后的炎症反应。按照 ISO 10993-6:2001 标准，以阵列形式将材料置于皮下，并监测宿主的反应。使用荧光全动物成像技术，在体内对这些材料的宿主细胞活性进行动态成像。使用全动物成像捕获的数据与组织学分析相比，显示出细胞对生物材料趋化的相似时间趋势。
结论/意义：组织学分析的相似性验证了这项技术是一种新颖的、快速的植入材料生物相容性筛选方法。通过这项技术，有可能在体内快速筛选大量聚合物文库。
