Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.

作者信息

Onuki-Nagasaki Reiko, Nagasaki Akira, Hakamada Kazumi, Uyeda Taro Q P, Fujita Satoshi, Miyake Masato, Miyake Jun

机构信息

Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.

出版信息

Methods Mol Biol. 2010;629:193-203. doi: 10.1007/978-1-60761-657-3_13.

DOI:10.1007/978-1-60761-657-3_13

PMID:20387151

Abstract

Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.

摘要