以细胞脲酶为标记物体外测定表皮葡萄球菌对塑料的黏附性。
In vitro measurement of the adherence of Staphylococcus epidermidis to plastic by using cellular urease as a marker.
作者信息
Dunne W M, Burd E M
机构信息
Department of Pathology, Children's Hospital of Wisconsin, Medical College of Wisconsin, Milwaukee 53201.
出版信息
Appl Environ Microbiol. 1991 Mar;57(3):863-6. doi: 10.1128/aem.57.3.863-866.1991.
A rapid and sensitive in vitro assay was developed to quantitatively assess the adherence of Staphylococcus epidermidis to a hydrophobic plastic surface. The assay is based upon the detection of cell-associated urease activity as a marker of bacteria remaining adherent to the polystyrene microwells of flat-bottomed, 96-well tissue culture plates. Using ATCC 35984, a slime-producing strain of S. epidermidis, the assay could detect as few as 3 x 10(3) bacteria and was linear to 3.5 x 10(7) bacteria. The adherence of both slime-positive and slime-negative coagulase-negative staphylococci could be evaluated by using this method. This assay could be used to examine factors which influence the adherence of individual S. epidermidis strains to hydrophobic surfaces and to develop agents or coating materials which suppress the adherence of coagulase-negative staphylococci to biomedical implants.
开发了一种快速灵敏的体外检测方法，用于定量评估表皮葡萄球菌对疏水塑料表面的黏附情况。该检测方法基于检测细胞相关脲酶活性，以此作为细菌仍黏附于平底96孔组织培养板聚苯乙烯微孔的标志物。使用表皮葡萄球菌的产黏液菌株ATCC 35984，该检测方法可检测低至3×10³个细菌，且在3.5×10⁷个细菌范围内呈线性关系。通过该方法可评估黏液阳性和黏液阴性凝固酶阴性葡萄球菌的黏附情况。此检测方法可用于研究影响单个表皮葡萄球菌菌株对疏水表面黏附的因素，以及开发抑制凝固酶阴性葡萄球菌黏附于生物医学植入物的试剂或涂层材料。
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