应用广谱细菌 16S rRNA 基因 PCR 技术检测培养阴性脑脓肿中的混合感染
Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR.
机构信息
University of Zurich, Institute of Medical Microbiology, Gloriastrasse 32, CH-8006 Zurich, Switzerland.
出版信息
J Clin Microbiol. 2010 Jun;48(6):2250-2. doi: 10.1128/JCM.01922-09. Epub 2010 Apr 14.
Abstract
We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.
摘要
我们通过使用广谱 16S rRNA 基因 PCR 鉴定了来自培养阴性脑脓肿的两种细菌病原体。由于它们部分扩增的 16S rRNA 基因存在 24 个碱基对的长度差异，因此可以同时检测到核梭杆菌和牙髓卟啉单胞菌，这使得它们可以通过高分辨率聚丙烯酰胺凝胶电泳分离。
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