Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Estatal Santa Ines Tecuexcomac-Tepetitla Km 1.5, Tepetitla, Tlaxcala, México, C.P. 70900.
Environ Sci Pollut Res Int. 2015 Jan;22(2):1034-42. doi: 10.1007/s11356-014-3357-y. Epub 2014 Aug 10.
Trichoderma asperellum H15, a previously isolated strain characterized by its high tolerance to low (LMW) and high molecular weight (HMW) PAHs, was tested for its ability to degrade 3-5 ring PAHs (phenanthrene, pyrene, and benzo[a]pyrene) in soil microcosms along with a biostimulation treatment with sugarcane bagasse. T. asperellum H15 rapidly adapted to PAH-contaminated soils, producing more CO2 than uncontaminated microcosms and achieving up to 78 % of phenanthrene degradation in soils contaminated with 1,000 mg Kg(-1) after 14 days. In soils contaminated with 1,000 mg Kg(-1) of a three-PAH mixture, strain H15 was shown to degrade 74 % phenanthrene, 63 % pyrene, and 81 % of benzo[a]pyrene. Fungal catechol 1,2 dioxygenase, laccase, and peroxidase enzyme activities were found to be involved in the degradation of PAHs by T. asperellum. The results demonstrated the potential of T. asperellum H15 to be used in a bioremediation process. This is the first report describing the involvement of T. asperellum in LMW and HMW-PAH degradation in soils. These findings, along with the ability to remove large amounts of PAHs in soil found in the present work provide enough evidence to consider T. asperellum as a promising and efficient PAH-degrading microorganism.
木霉asperellum H15，是一株先前被分离出来的菌株，具有较高的耐低分子量（LMW）和高分子量（HMW）多环芳烃（PAHs）的特性，被测试其在土壤微宇宙中降解 3-5 环 PAHs（菲、芘和苯并[a]芘）的能力，同时还进行了用甘蔗渣进行生物刺激处理。T. asperellum H15 能快速适应受 PAH 污染的土壤，产生的 CO2 比未受污染的微宇宙多，在受 1000 mg Kg(-1)污染的土壤中，经过 14 天，达到了 78%的菲降解率。在受 1000 mg Kg(-1)的三种 PAH 混合物污染的土壤中，菌株 H15 被证明可以降解 74%的菲、63%的芘和 81%的苯并[a]芘。发现真菌儿茶酚 1,2 双加氧酶、漆酶和过氧化物酶的酶活性参与了 T. asperellum 对 PAHs 的降解。结果表明，T. asperellum H15 有潜力用于生物修复过程。这是第一个描述 T. asperellum 参与土壤中 LMW 和 HMW-PAH 降解的报告。这些发现，以及本工作中发现的在土壤中去除大量 PAHs 的能力，提供了足够的证据，可以考虑 T. asperellum 作为一种有前途和高效的 PAH 降解微生物。
