Watson C K, Cole J R, Pursell A R
Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, Tifton 31794.
J Vet Diagn Invest. 1991 Jan;3(1):66-71. doi: 10.1177/104063879100300114.
The correlation between the antimicrobic standard disk agar diffusion procedure and a veterinary antimicrobic breakpoint minimal inhibitory concentration system was evaluated. Bacterial isolates representing 5 different genera were tested against 15 antimicrobics. There were 3,795 tests performed; 77.3% of the test results were in agreement, 22.6% were in disagreement. Thirty-one percent of the conflicting results were due to the organism being intermediate on the standard disk agar diffusion procedure. Results suggest that the breakpoint system needs more than 1 antimicrobic dilution per antimicrobic, or a change in some of the single dilutions in the breakpoint system would eliminate some of these discrepancies. Another possibility would be to utilize more than 1 panel design, i.e., 1 for gram-positive organisms, 1 for gram-negative organisms, and 1 for fastidious organisms.
评估了抗菌标准纸片琼脂扩散法与兽医抗菌药物折点最低抑菌浓度系统之间的相关性。对代表5个不同属的细菌分离株进行了15种抗菌药物的测试。共进行了3795次测试；77.3%的测试结果一致，22.6%不一致。31%的冲突结果是由于该微生物在标准纸片琼脂扩散法中处于中介状态。结果表明，折点系统每种抗菌药物需要不止一种抗菌药物稀释度，或者改变折点系统中的一些单一稀释度将消除一些差异。另一种可能性是采用不止一种平板设计，即一种用于革兰氏阳性菌，一种用于革兰氏阴性菌，一种用于苛养菌。
