Stubbs K G, Wicher K
Am J Clin Pathol. 1977 Dec;68(6):769-777. doi: 10.1093/ajcp/68.6.769.
A newly introduced automated method for antibiotic susceptibility testing, AUTOBAC 1, has been evaluated by comparison with the disk agar diffusion method (Bauer-Kirby). A total of 2,518 strains of gram-positive (540) and gram-negative (1,978) organisms isolated from clinical specimens was examined by both methods with eight or ten antibiotics, including Tobramycin. An overall agreement of 97.4% was obtained when results were compared by individual antibiotic. However, many discrepancies were observed when individual genera or species were analyzed. Of 2,518 strains examined, 651 (26%) showed discrepancies in response to one or more antibiotics. Strains, showing discrepancies were re-examined by the broth dilution susceptibility method. The results obtained favored the disk agar diffusion method. Reproducibility experiments revealed a greater inconsistency in the AUTOBAC 1 system than in the agar diffusion test. It is concluded that although a rapid automated system for antibiotic sensitivity testing is desirable, the conventional disk agar diffusion method is easier to perform, more reliable, and a less expensive procedure for antibiotic sensitivity determination.
一种新引入的抗生素敏感性检测自动化方法AUTOBAC 1，已通过与纸片琼脂扩散法（鲍尔-柯比法）进行比较来评估。使用包括妥布霉素在内的8种或10种抗生素，通过这两种方法对总共2518株从临床标本中分离出的革兰氏阳性菌（540株）和革兰氏阴性菌（1978株）进行了检测。按单一抗生素比较结果时，总体一致性为97.4%。然而，在分析各个属或种时，观察到许多差异。在所检测的2518株菌株中，651株（26%）对一种或多种抗生素的反应显示出差异。对显示出差异的菌株通过肉汤稀释敏感性方法重新检测。所得结果更支持纸片琼脂扩散法。重复性实验表明，AUTOBAC 1系统比琼脂扩散试验的不一致性更大。结论是，虽然需要一种快速的抗生素敏感性检测自动化系统，但传统的纸片琼脂扩散法操作更简便、更可靠，且在抗生素敏感性测定方面成本更低。
