Baker C N, Stocker S A, Culver D H, Thornsberry C
Antimicrobic Investigations Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1991 Mar;29(3):533-8. doi: 10.1128/jcm.29.3.533-538.1991.
The E Test (AB Biodisk, Solna, Sweden) is a new method for performing antimicrobial susceptibility tests. It consists of an impervious carrier (5- by 50-mm strip) with a predefined antimicrobic gradient which is placed on an inoculated agar plate and processed like a disk diffusion test. Results are generated directly as MICs from a continuous concentration gradient covering 15 twofold dilutions, and MICs are read where the edge of the inhibition zone intersects the strip. We compared the E Test with disk diffusion, broth microdilution, and agar dilution tests by using a challenge set of 195 gram-positive and gram-negative bacteria for 14 antimicrobial agents. Also, disk diffusion, broth microdilution, and agar dilution tests were compared with each other. All test method comparisons gave greater than 94% agreement for the category of susceptibility. The E Test category agreement with disk diffusion and broth microdilution was 95.1%, and with agar dilution it was 95.2%. The E Test results were as reliable as the results obtained by the standard antimicrobial susceptibility testing methods.
E试验（AB生物盘公司，瑞典索尔纳）是一种进行抗菌药敏试验的新方法。它由一个不透水的载体（5×50毫米的试纸条）组成，带有预定义的抗菌药物梯度，放置在接种的琼脂平板上，其操作过程类似于纸片扩散试验。结果直接以最低抑菌浓度（MIC）的形式产生，该浓度来自覆盖15个两倍稀释度的连续浓度梯度，MIC值在抑菌圈边缘与试纸条相交处读取。我们使用一组包含195株革兰氏阳性和革兰氏阴性细菌的菌株，针对14种抗菌药物，将E试验与纸片扩散法、肉汤微量稀释法和琼脂稀释法进行了比较。此外，还对纸片扩散法、肉汤微量稀释法和琼脂稀释法进行了相互比较。所有试验方法在药敏类别方面的一致性均超过94%。E试验与纸片扩散法和肉汤微量稀释法在类别上的一致性为95.1%，与琼脂稀释法的一致性为95.2%。E试验的结果与标准抗菌药敏试验方法所获得的结果一样可靠。
