Gustinelli Andrea, Caffara Monica, Florio Daniela, Otachi Elick O, Wathuta Euty M, Fioravanti Maria L
Department of Veterinary Public Health and Animal Pathology, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy.
Syst Parasitol. 2010 May;76(1):39-51. doi: 10.1007/s11230-010-9231-5. Epub 2010 Apr 17.
The combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of Clinostomum cutaneum Paperna, 1964 from the grey heron Ardea cinerea L. in Kenya. A redescription of the metacercaria that infect Nile tilapia Oreochromis niloticus niloticus (L.) from the same aquatic environment is also presented. C. cutaneum differs from all other species of Clinostomum Leidy, 1856 in the shape of its uterus. Sequencing the rRNA confirmed the morphological similarity between adults from the grey heron and the metacercarial stage from tilapia, and a level of genetic similarity with the other previously sequenced Clinostomum spp. was observed. The need for a reorganisation of Clinostomum using both morphological and molecular methods is highlighted.
形态学和分子研究的联合运用首次使得人们能够识别并描述1964年帕佩纳在肯尼亚的灰鹭(苍鹭)身上发现的皮肤斜睾吸虫的成虫阶段。本文还对来自同一水生环境中感染尼罗罗非鱼(尼罗罗非鱼)的囊蚴进行了重新描述。皮肤斜睾吸虫在子宫形态上与1856年利迪描述的斜睾属的所有其他物种不同。对核糖体RNA进行测序证实了灰鹭体内的成虫与罗非鱼体内的囊蚴阶段在形态上具有相似性,并且观察到与其他先前测序的斜睾属物种存在一定程度的基因相似性。强调了使用形态学和分子方法对斜睾属进行重新分类的必要性。