Division of Functional Morphology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan.
Histochem Cell Biol. 2010 Jun;133(6):627-39. doi: 10.1007/s00418-010-0699-5. Epub 2010 Apr 18.
The localization of vasa homolog protein in the spermatogenic cells of mice, rats, and guinea pigs was studied by immunofluorescence and electron microscopies with the antibody against mouse vasa homolog (MVH) protein. By immunofluorescence microscopy, four types of granular staining patterns were identified: (1) fine particles observed in diplotene and meiotic cells, (2) small granules associated with a mitochondrial marker and appearing in pachytene spermatocytes after stage V, (3) strands lacking the mitochondrial marker in late spermatocytes, and (4) large irregularly shaped granules in round spermatids. Immunoelectron microscopy defined the ultrastructural profiles of these MVH protein-positive granules: the first type consisted of small dense particles, the second had intermitochondrial cement (IMC), the third type, consisting of strands, had loose aggregates of either material dissociated from IMC or 70-90-nm particles, and the fourth had typical chromatoid bodies (CBs). The results suggest that MVH proteins function in these components of nuage. MVH protein-positive structures other than CBs disappeared during meiosis and CB appeared first in early spermatids. The results suggest that the formation of nuage is discontinued between spermatocytes and spermatids. The formation of nuage in spermatocytes and of CB in spermatids is discussed.
用抗鼠 vasa 同源蛋白（MVH）抗体通过免疫荧光和电子显微镜研究了 vasa 同源蛋白在小鼠、大鼠和豚鼠生精细胞中的定位。通过免疫荧光显微镜，鉴定出四种颗粒状染色模式：（1）在偶线期和减数分裂细胞中观察到的细颗粒，（2）与线粒体标记物相关联并在 V 期后出现于粗线期精母细胞中的小颗粒，（3）在晚期精母细胞中缺乏线粒体标记物的链状，（4）在圆形精子中呈大的不规则形状的颗粒。免疫电子显微镜定义了这些 MVH 蛋白阳性颗粒的超微结构特征：第一类由小的致密颗粒组成，第二类有间质连接（IMC），第三类由链组成，由从 IMC 解离的松散聚集物或 70-90nm 的颗粒组成，第四类有典型的染色质体（CB）。结果表明，MVH 蛋白在这些核质体成分中发挥作用。减数分裂期间除 CB 以外的 MVH 蛋白阳性结构消失，而 CB 最早出现在早期精子中。结果表明核质体的形成在精母细胞和精子之间停止。讨论了精母细胞中核质体的形成和精子中 CB 的形成。
