Cid14 非典型多聚（A）聚合酶的蛋白质组学和功能分析。
Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14.
机构信息
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
出版信息
RNA. 2010 Jun;16(6):1124-9. doi: 10.1261/rna.2053710. Epub 2010 Apr 19.
Abstract
The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in a broad range of biological functions. Here we describe an extensive Cid14 protein-protein interaction network and its biochemical dissection. Cid14 most stably interacts with the zinc-knuckle protein Air1 to form the Cid14-Air1 complex (CAC). Providing a link to ribosomal RNA processing, Cid14 sediments with 60S ribosomal subunits and copurifies with 60S assembly factors. In contrast, no physical link to chromatin has been identified, although gene expression profiling revealed that efficient silencing of a few heterochromatic genes depends on Cid14 and/or Air1.
摘要
裂殖酵母 Cid14 蛋白属于一类非典型多聚（A）聚合酶家族，该家族与多种生物学功能有关。在此，我们描述了一个广泛的 Cid14 蛋白-蛋白相互作用网络及其生化解析。Cid14 最稳定地与锌指蛋白 Air1 相互作用，形成 Cid14-Air1 复合物（CAC）。Cid14 通过与核糖体 RNA 加工相联系，与 60S 核糖体亚基共沉淀，并与 60S 组装因子共纯化。相比之下，尚未发现与染色质的物理联系，尽管基因表达谱分析显示，少数异染色质基因的有效沉默依赖于 Cid14 和/或 Air1。
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