Department of Genetics and Pathology, Uppsala University, Uppsala S-75185, Sweden.
Genome Res. 2010 Jul;20(7):899-907. doi: 10.1101/gr.103473.109. Epub 2010 Apr 19.
Noncoding RNA (ncRNA) constitutes a significant portion of the mammalian transcriptome. Emerging evidence suggests that it regulates gene expression in cis or trans by modulating the chromatin structure. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harboring CARs. The intronic and intergenic CARs show significant conservation across 44 species of placental mammals. Functional characterization of one of the intergenic CARs, Intergenic10, revealed that it regulates gene expression of neighboring genes through modulating the chromatin structure in cis. Our data suggest that ncRNA is an integral component of chromatin and that it may regulate various biological functions through fine-tuning of the chromatin architecture.
非编码 RNA（ncRNA）构成了哺乳动物转录组的重要部分。新出现的证据表明，它通过调节染色质结构来在顺式或反式上调节基因表达。为了揭示 ncRNA 在染色质组织中的功能作用，我们对人类成纤维细胞（HF）中的染色质相关 RNA（CAR）进行了深度测序。这导致了 141 个内含子区域和 74 个基因间区域含有 CAR 的鉴定。内含子和基因间 CAR 在 44 种胎盘哺乳动物中具有显著的保守性。对一个基因间 CAR，Intergenic10 的功能表征表明，它通过在顺式调节染色质结构来调节邻近基因的表达。我们的数据表明，ncRNA 是染色质的一个组成部分，它可能通过精细调节染色质结构来调节各种生物学功能。
