从临床样本中分离出的金黄色葡萄球菌中的可诱导克林霉素耐药性。
Inducible clindamycin resistance in Staphylococcus aureus isolated from clinical samples.
作者信息
Deotale V, Mendiratta D K, Raut U, Narang P
机构信息
Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences, Sevagram. Dist Wardha, M.S. - 442 102, India.
出版信息
Indian J Med Microbiol. 2010 Apr-Jun;28(2):124-6. doi: 10.4103/0255-0857.62488.
PURPOSE
Clindamycin is commonly used in the treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to erm genes resulting in treatment failure, thus necessitating the need to detect such resistance by a simple D test on routine basis.
MATERIALS AND METHOD
247 Staphylococcus aureus isolates were subjected to routine antibiotic susceptibility testing including oxacillin (1ìg) by Kirby Bauer disc diffusion method. Inducible clindamycin resistance was detected by D test, as per CLSI guidelines on erythromycin resistant isolates.
RESULTS
36 (14.5%) isolates showed inducible clindamycin resistance, nine (3.6%) showed constitutive resistance while remaining 35 (14.1%) showed MS phenotype. Inducible resistance and MS phenotype were found to be higher in MRSA as compared to MSSA (27.6%, 24.3% and 1.6%, 4% respectively).
CONCLUSION
Study showed that D test should be used as a mandatory method in routine disc diffusion testing to detect inducible clindamycin resistance.
目的
克林霉素常用于治疗由耐红霉素金黄色葡萄球菌引起的皮肤和软组织感染。由于erm基因导致的诱导型克林霉素耐药，克林霉素敏感性的体外常规检测可能无法检测到，从而导致治疗失败，因此有必要在常规基础上通过简单的D试验检测此类耐药性。
材料与方法
采用 Kirby Bauer 纸片扩散法，对247株金黄色葡萄球菌分离株进行包括苯唑西林（1μg）在内的常规抗生素敏感性试验。根据CLSI关于耐红霉素分离株的指南，通过D试验检测诱导型克林霉素耐药性。
结果
36株（14.5%）分离株表现出诱导型克林霉素耐药，9株（3.6%）表现出组成型耐药，其余35株（14.1%）表现出MS表型。与MSSA相比，MRSA中诱导型耐药和MS表型更高（分别为27.6%、24.3%和1.6%、4%）。
结论
研究表明，在常规纸片扩散试验中，应使用D试验作为检测诱导型克林霉素耐药性的强制方法。
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