组蛋白 H4 赖氨酸 20 单甲基化和三甲基化定义了 SV40 微小染色体中的不同生物学过程。

Histone H4 lysine 20 mono- and tri-methylation define distinct biological processes in SV40 minichromosomes.

机构信息

Department of Biochemistry and Molecular Biology, University of North Dakota, Grand Forks, ND, USA.

出版信息

Cell Cycle. 2010 Apr 1;9(7):1320-32. doi: 10.4161/cc.9.7.11123.

DOI:10.4161/cc.9.7.11123

PMID:20404477

Abstract

The methylation profile of histone h4 on lysine 20 in sV40 chromatin during an infection was investigated using ChIp analyses with antibodies to monomethyl (h4K20me1), dimethyl (h4K20me2), and trimethyl (h4K20me3) histone h4. h4K20me1 was found in late-transcribing, uncoating, encapsidating and replicating minichromosomes as well as in the sV40 chromatin present in virions. Its prevalence was greatest in virions and least in minichromosomes present between 4 and 24 hours post-infection. In contrast, h4K20me2 did not appear to be present and h4K20me3 appeared to be present only in minichromosomes obtained 30 minutes post-infection. The presence of h4K20me1 late in infection in replicating minichromosomes and its relative enrichment in virions suggested that it played a role in the encapsidation process. In contrast, the presence of h4K20me3 at the earliest stages of the infection and its subsequent relatively rapid loss along with sV40 chromatin suggested that it was functioning during the uncoating process

摘要

在 S V40 感染过程中，利用针对单甲基化（h4K20me1）、二甲基化（h4K20me2）和三甲基化（h4K20me3）组蛋白 h4 的 ChIP 分析，研究了 S V40 染色质组蛋白 h4 赖氨酸 20 上组蛋白 h4 的甲基化谱。在晚期转录、脱壳、封装和复制的小染色体以及病毒粒子中的 S V40 染色质中发现了 h4K20me1。其丰度在病毒粒子中最高，在感染后 4 至 24 小时之间存在的小染色体中最低。相比之下，h4K20me2 似乎不存在，而 h4K20me3 似乎仅存在于感染后 30 分钟获得的小染色体中。感染后期复制小染色体中 h4K20me1 的存在及其在病毒粒子中的相对富集表明，它在封装过程中发挥了作用。相比之下，h4K20me3 在感染的最早阶段存在，随后随着 S V40 染色质的迅速丢失，表明它在脱壳过程中发挥了作用。