Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea.
Natural Product Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Korea.
Int J Mol Sci. 2020 Feb 20;21(4):1435. doi: 10.3390/ijms21041435.
We investigated the metabolite changes of roots (MRs) according to different cultivar families (, , , , , , , and ) using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) to understand the relationship between different cultivars and metabolite changes. Data were analyzed by partial least squares discriminant analysis (PLS-DA), and samples were successfully separated in PLS-DA scores. Eight metabolites in the electrospray ionization (ESI)-positive mode and 16 metabolites in the ESI-negative mode contributed to the separation in PLS-DA. Our data suggest that comparative analysis of MR metabolites according to different cultivars is useful to better understand the relationship between the different cultivars and metabolite changes. Furthermore, we analyzed the MRs for their ability to improve benign prostatic hyperplasia (BPH). LNCaP cells were used to evaluate the prostate-specific antigen (PSA) inhibitory activity of MRs, and, amongst them, the extract with the highest activity was selected. demonstrated the highest inhibition effect of prostate-specific antigen (PSA) expression among the MR cultivars. was also evaluated by administration in a testosterone-induced benign prostatic hyperplasia model in Sprague-Dawley rats. was shown to ameliorate BPH as evidenced by the prostate index, expression of androgen receptor (AR) signaling-related protein, growth factors, cell proliferation-related proteins, apoptosis-related proteins, mitogen-activated protein kinase (MAPK) signaling proteins, and histological analysis. Hence, this study strongly suggests that may have a beneficial effect of on BPH.
我们使用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS）研究了不同品种（、、、、、、和）根（MRs）的代谢物变化，以了解不同品种之间的关系和代谢物变化。数据通过偏最小二乘判别分析（PLS-DA）进行分析，并且在 PLS-DA 得分中成功分离了样品。电喷雾电离（ESI）正模式下的 8 种代谢物和 ESI 负模式下的 16 种代谢物对 PLS-DA 的分离有贡献。我们的数据表明，根据不同品种对 MR 代谢物进行比较分析有助于更好地理解不同品种之间的关系和代谢物变化。此外，我们分析了 MRs 改善良性前列腺增生（BPH）的能力。LNCaP 细胞用于评估 MRs 对前列腺特异性抗原（PSA）抑制活性，其中具有最高活性的提取物被选中。在 MR 品种中，表现出对前列腺特异性抗原（PSA）表达的最高抑制作用。在去势雄性大鼠的睾酮诱导的良性前列腺增生模型中还评价了 。结果表明，通过前列腺指数、雄激素受体（AR）信号相关蛋白、生长因子、细胞增殖相关蛋白、凋亡相关蛋白、丝裂原激活蛋白激酶（MAPK）信号蛋白的表达以及组织学分析，改善了 BPH。因此，这项研究强烈表明 可能对 BPH 具有有益的作用。
