Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China.
Emerg Infect Dis. 2010 May;16(5):764-8. doi: 10.3201/eid1605.021293.
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
为了研究中国野生动物和家畜中的嗜吞噬细胞无形体菌株，我们从中国东北地区的啮齿动物和绵羊中分离出这种病原体。我们通过在 BALB/c 小鼠中繁殖，然后在 HL60 细胞中培养，从啮齿动物和患病绵羊中分离出 3 株（2 株来自啮齿动物，1 株来自患病绵羊）。通过瑞氏染色、免疫荧光和电子显微镜鉴定了这 3 株分离株，并通过 16S rRNA 基因、部分柠檬酸合酶基因、主要表面蛋白 4 基因和热休克蛋白基因的序列分析进行了特征描述。这 3 株分离株的多个序列彼此相同，但与来自其他国家的所有已知菌株不同。这些分离株的公共卫生和兽医相关性值得进一步研究。
