Hillyer G V, Soler de Galanes M
Department of Pathology, University of Puerto Rico School of Medicine, San Juan 00936-5067.
J Parasitol. 1991 Jun;77(3):362-5.
The Falcon assay screening test enzyme-linked immunosorbent assay was adapted for the detection of antibodies to Fasciola hepatica excretion-secretion (FhES) antigens in various animal models. Pooled serum from 5 5-wk-old sheep infected with 400 F. hepatica metacercariae had high absorbance levels by 2 wk of infection and rose again at 8-10 wk. Pooled serum from 5 6-wk-old Holstein calves infected with 700 F. hepatica metacercariae had an increase in absorbance levels by 2 wk of infection, rising through 6 wk of infection. Rabbits with a primary F. hepatica infection (6-7 worms) developed antibodies to FhES by 3 wk of infection, peaking by 5 wk and remaining at high levels through the 16 wk tested. Mice with a primary F. hepatica infection developed antibodies to FhES rapidly, rising by 1 wk of infection and peaking 1-3 wk later. The sera from mice with a primary Schistosoma mansoni infection were also examined for the production of antibodies to both S. mansoni worm antigens (SmWWE) and to FhES. Antibodies to SmWWE rose by 5 wk of infection, peaking 1-3 wk later; the antibody levels to FhES rose at 6 wk with the absorbance values peaking 1 wk later and were always lower than those to SmWWE. This suggests that the anti-FhES antibodies in murine schistosomiasis mansoni may be due to cross-reactive antibodies to S. mansoni egg antigens.
将猎鹰分析筛选试验酶联免疫吸附测定法进行改良，用于检测各种动物模型中针对肝片吸虫排泄-分泌（FhES）抗原的抗体。来自5只感染400个肝片吸虫囊蚴的5周龄绵羊的混合血清，在感染2周时吸光度水平较高，并在8至10周时再次升高。来自5只感染700个肝片吸虫囊蚴的6周龄荷斯坦犊牛的混合血清，在感染2周时吸光度水平增加，在感染6周期间持续上升。初次感染肝片吸虫（6至7条虫）的兔子在感染3周时产生了针对FhES的抗体，在5周时达到峰值，并在测试的16周内一直保持在高水平。初次感染肝片吸虫的小鼠迅速产生针对FhES的抗体，在感染1周时升高，并在1至3周后达到峰值。还检测了初次感染曼氏血吸虫的小鼠血清中针对曼氏血吸虫虫体抗原（SmWWE）和FhES的抗体产生情况。针对SmWWE的抗体在感染5周时升高，在1至3周后达到峰值；针对FhES的抗体在6周时升高，吸光度值在1周后达到峰值，且始终低于针对SmWWE的抗体水平。这表明曼氏血吸虫病小鼠中的抗FhES抗体可能是由于对曼氏血吸虫卵抗原的交叉反应性抗体所致。
