建立一种检测鸭圆环病毒感染的间接 ELISA 方法。
Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks.
机构信息
Laboratory of Taishan Scholar Immunobiology, China.
出版信息
Vet Microbiol. 2010 Sep 28;145(1-2):41-6. doi: 10.1016/j.vetmic.2010.03.010. Epub 2010 Mar 17.
Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by cloning the cap (Cap) gene of DuCV FJ0601 strain into pET-32a (+) vector and expressed in Escherichia coli. Using the optimized iELISA method, DuCV-specific antibodies were detected in 157 (12.96%) of 1211 samples obtained from 17 (89.47%) of 19 meat duck flocks aged from 25 to 40 days and in 89 (22.08%) of 403 samples obtained from 9 (75%) of 12 breeder flocks aged from 14 to 61 weeks. These results indicated that the iELISA method is useful for serological diagnosis of DuCV infection and epidemiological investigation.
鸭圆环病毒（DuCV）感染与养殖鸭的生长迟缓及发育问题有关。为了检测鸭血清中的 DuCV 特异性抗体，本研究通过将 DuCV FJ0601 株的 Cap 基因克隆到 pET-32a（+）载体中，并在大肠杆菌中表达，制备了重组衣壳蛋白抗原，建立了间接酶联免疫吸附试验（iELISA）方法。利用优化后的 iELISA 方法，在来自 25 至 40 日龄的 17 个（89.47%）肉鸭群的 1211 个样本中检测到 157 个（12.96%）样本，以及在来自 14 至 61 周龄的 9 个（75%）种鸭群的 403 个样本中检测到 89 个（22.08%）样本中，均检测到了 DuCV 特异性抗体。这些结果表明，该 iELISA 方法可用于 DuCV 感染的血清学诊断和流行病学调查。
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