Institute of Biomedical and Clinical Science, Peninsula Medical School, University of Exeter, St Luke's Campus, Magdalen Road, Exeter, Devon EX1 2LU, UK.
Diabetologia. 2010 Aug;53(8):1722-6. doi: 10.1007/s00125-010-1761-5. Epub 2010 Apr 23.
AIMS/HYPOTHESIS: Hydrogen sulphide is a recently identified endogenous endothelium-dependent vasodilator. Animal models of diabetes have shown that low plasma H(2)S levels are associated with marked endothelial dysfunction and insulin resistance. However, human studies on H(2)S and vascular function in health and disease are lacking.
Plasma was obtained from male patients with type 2 diabetes (n = 11), overweight (n = 16) and lean (n = 11) volunteers. H(2)S levels were determined by zinc trap spectrophotometry. Anthropometric measurements (BMI/waist:hip ratio), lipid profile, systemic blood pressure, biochemical indices of diabetes (fasting glucose, insulin sensitivity, Hb(1Ac)) and microvascular function (minimum vascular resistance) were determined.
Median plasma H(2)S levels (25th, 75th percentiles) in age-matched lean, overweight and type 2 diabetes individuals were 38.9 (29.7, 45.1) micromol/l, 22.0 (18.6, 26.7) micromol/l and 10.5 (4.8, 22.0) micromol/l, respectively. Median plasma H(2)S levels were significantly lower in patients with type 2 diabetes compared with lean (p = 0.001, Mann-Whitney) and overweight participants (p = 0.008). Median plasma H(2)S levels in overweight participants were significantly lower than in lean controls (p = 0.003). Waist circumference was an independent predictor of plasma H(2)S (R (2) = 0.423, standardised beta: -0.650, p < 0.001). This relationship was independent of diabetes, which only contributed a further 5% to the model (R (2) = 0.477). Waist circumference or other measures of adiposity (waist:hip ratio/BMI) remained independent predictors of plasma H(2)S after adjustment for systolic blood pressure, microvascular function, insulin sensitivity, glycaemic control and lipid profile.
CONCLUSIONS/INTERPRETATION: Plasma H(2)S levels are reduced in overweight participants and patients with type 2 diabetes. Increasing adiposity is a major determinant of plasma H(2)S levels.
目的/假设：硫化氢是一种新发现的内源性内皮依赖性血管舒张剂。糖尿病动物模型表明，血浆 H(2)S 水平降低与明显的内皮功能障碍和胰岛素抵抗有关。然而，在健康和疾病状态下，人类关于 H(2)S 和血管功能的研究还很缺乏。
从男性 2 型糖尿病患者（n=11）、超重（n=16）和瘦（n=11）志愿者中获得血浆。通过锌陷阱分光光度法测定 H(2)S 水平。测定人体测量学指标（BMI/腰围：臀围比）、血脂谱、全身血压、糖尿病的生化指标（空腹血糖、胰岛素敏感性、Hb(1Ac))和微血管功能（最小血管阻力）。
年龄匹配的瘦、超重和 2 型糖尿病个体的中位数血浆 H(2)S 水平（25%，75%）分别为 38.9（29.7，45.1）µmol/l、22.0（18.6，26.7）µmol/l和 10.5（4.8，22.0）µmol/l。与瘦对照组（p=0.001，Mann-Whitney）和超重参与者（p=0.008）相比，2 型糖尿病患者的中位血浆 H(2)S 水平显著降低。超重参与者的中位血浆 H(2)S 水平明显低于瘦对照组（p=0.003）。腰围是血浆 H(2)S 的独立预测因子（R (2) = 0.423，标准化β：-0.650，p < 0.001）。这一关系独立于糖尿病，糖尿病仅对模型的进一步贡献了 5%（R (2) = 0.477）。调整收缩压、微血管功能、胰岛素敏感性、血糖控制和血脂谱后，腰围或其他肥胖指标（腰围：臀围比/BMI）仍然是血浆 H(2)S 的独立预测因子。
结论/解释：超重参与者和 2 型糖尿病患者的血浆 H(2)S 水平降低。肥胖程度的增加是血浆 H(2)S 水平的主要决定因素。
