罗氏 COBAS 6000 分析仪上胶乳增强散射比浊法测定胱抑素 C 的分析性能评估。
Analytical performance evaluation of a particle-enhanced turbidimetric cystatin C assay on the Roche COBAS 6000 analyzer.
机构信息
U.G.C. Laboratorio Clínico, Hospital Nuestra Señora de la Merced, Avda. De la Constitución, 2. 41640-Osuna, Seville, Spain.
出版信息
Clin Biochem. 2010 Jul;43(10-11):921-5. doi: 10.1016/j.clinbiochem.2010.04.057. Epub 2010 Apr 21.
OBJECTIVES
Cystatin C is a low molecular protein that has been proposed to estimate the glomerular filtration rate. Here we investigated the performance of the Roche cystatin C assay on the COBAS 6000 analyzer.
DESIGN AND METHODS
We studied the imprecision, recovery, limit of detection and quantification, linearity and interferences. For method comparison, split sample aliquots were assayed using the described method and a Siemens cystatin C assay.
RESULTS
The assay displayed a low total imprecision and a good linearity over the entire range tested. Bilirubin and triglycerides did not interfere with the assay, and only a haemoglobin concentration higher than 6g/dl interfered with the assay. The assay agreed well with the Siemens assay.
CONCLUSION
The Roche cystatin C assay is an acceptable method for determining the cystatin C and the glomerular filtration rate estimate. On a COBAS 6000, the assay improves and simplifies the laboratory's workload.
目的
胱抑素 C 是一种低分子量蛋白，据提议可用于估算肾小球滤过率。在此，我们对罗氏胱抑素 C 检测法在 COBAS 6000 分析仪上的性能进行了研究。
设计和方法
我们研究了不精密度、回收率、检测限和定量限、线性度和干扰。为了方法比较，使用描述的方法和西门子胱抑素 C 检测法对分拆的样本等分试样进行检测。
结果
该检测法显示出较低的总不精密度和在整个测试范围内的良好线性度。胆红素和甘油三酯对检测法无干扰，仅血红蛋白浓度高于 6g/dl 时对检测法有干扰。该检测法与西门子检测法吻合良好。
结论
罗氏胱抑素 C 检测法是一种可接受的方法，可用于确定胱抑素 C 和估算肾小球滤过率。在 COBAS 6000 上，该检测法可改善和简化实验室的工作量。
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