Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Int J Oral Maxillofac Surg. 2010 Jun;39(6):599-605. doi: 10.1016/j.ijom.2010.02.024. Epub 2010 Apr 24.
Twenty traditional Chinese medicines (TCM) were evaluated for their antimicrobial activity against four common oral bacteria. TCMs were tested for sensitivity against Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis. Aliquots of suspension of each bacterial species were inoculated onto a horse blood agar plate with TCMs soaked separately on 6mm paper disks. The plates were incubated for 48h anaerobically and the mean diameters of growth inhibition of three different areas obtained. 0.2% (w/v) chlorhexidine was used as a positive control. Broth microdilution assay was used to determine minimum inhibitory concentration and minimum bactericidal concentration. Fructus armeniaca mume was effective against all four bacteria. Thirteen TCMs demonstrated antimicrobial activity against Porphyromonas gingivalis, including Cortex magnoliae officinalis, Cortex phellodendri, Flos caryophylli, Flos lonicerae japonicae, Fructus armeniaca mume, Fructus forsythiae suspensae, Herba cum radice violae yedoensitis, Herba menthae haplocalycis, Pericarpium granati, Radix et rhizoma rhei, Radix gentianae, Ramulus cinnamomi cassia and Rhizoma cimicifugae. Cortex phellodendri showed antimicrobial activity against Streptococcus mutans, while Radix et rhizoma rhei was effective against Streptococcus mitis and Streptococcus sanguis. Fructus armeniaca mume had inhibitory effects against Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis in vitro.
20 种中药（TCM）被评估了对四种常见口腔细菌的抗菌活性。TCM 被测试了对变异链球菌、血链球菌、变形链球菌和牙龈卟啉单胞菌的敏感性。每种细菌的悬浮液等分试样接种到含有单独浸泡在 6mm 药敏纸片上的 TCM 的马血琼脂板上。平板在厌氧菌中孵育 48 小时，并获得三个不同区域的生长抑制的平均直径。0.2%（w/v）洗必泰被用作阳性对照。肉汤微量稀释法用于确定最小抑菌浓度和最小杀菌浓度。乌梅对所有四种细菌均有效。13 种 TCM 对牙龈卟啉单胞菌具有抗菌活性，包括厚朴、黄柏、丁香、金银花、乌梅、连翘、紫花地丁、薄荷、石榴皮、大黄、秦艽、肉桂和川芎。黄柏对变形链球菌具有抗菌活性，而大黄对变异链球菌和血链球菌有效。乌梅对变异链球菌、血链球菌、变形链球菌和牙龈卟啉单胞菌具有体外抑制作用。
