Department of Orthopaedics, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China.
Brain Res. 2010 Jun 25;1342:1-10. doi: 10.1016/j.brainres.2010.04.038. Epub 2010 Apr 25.
The current study was performed to investigate the effect of aminoguanidine (AG) on spinal cord injury (SCI) in rat. AG (75, 150 and 300mg/kg, i.p. respectively ) was administered to rats immediately following SCI. It was found that AG (150mg/kg) significantly reduced spinal cord water content and improved motor function, however, AG at the doses of 75 and 300mg/kg had no effect. Compared to SCI group without treatment, AG at the dosage of 150mg/kg induced a reduction in the permeability of blood-spinal cord barrier (BSCB) after injury 48h (from 59.8+/-5.5microl/g to 39.8+/-3.8microl/g), a 38% decrease of Malondialdehyde (MDA) values and a 1-fold increase of the Glutathione (GSH) levels at 12h after SCI. And the expression of inducible nitric oxide synthase (iNOS) protein reached a peak at 24h after injury, which was significantly attenuated by treatment with AG (150mg/kg). In addition, the expression of AQP4 protein was down-regulated by the treatment of AG (150mg/kg) at 24h after SCI, and the changes still lasted at 48h after injury. Our results indicated that AG could induce spinal cord edema clearance and improve motor function, which could be correlated with antioxidative property, the down-regulation of iNOS and AQP4 protein expression after SCI.
本研究旨在探讨氨基胍（AG）对大鼠脊髓损伤（SCI）的影响。AG（分别为 75、150 和 300mg/kg，腹腔注射）在 SCI 后立即给予大鼠。结果发现，AG（150mg/kg）显著降低脊髓含水量，改善运动功能，但 AG 的剂量为 75 和 300mg/kg 时则没有效果。与未经治疗的 SCI 组相比，AG （150mg/kg）在损伤后 48h 时可降低血脊髓屏障（BSCB）的通透性（从 59.8+/-5.5μl/g 降至 39.8+/-3.8μl/g），减少丙二醛（MDA）值的 38%，增加谷胱甘肽（GSH）水平的 1 倍SCI 后 12h。诱导型一氧化氮合酶（iNOS）蛋白的表达在损伤后 24h 达到峰值，用 AG（150mg/kg）治疗可显著减弱其表达。此外，AG（150mg/kg）治疗可在 SCI 后 24h 下调 AQP4 蛋白的表达，且这种变化在损伤后 48h 仍持续存在。我们的结果表明，AG 可诱导脊髓水肿清除和改善运动功能，这可能与抗氧化特性、SCI 后 iNOS 和 AQP4 蛋白表达下调有关。
