Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Metab Brain Dis. 2010 Jun;25(2):219-25. doi: 10.1007/s11011-010-9187-9. Epub 2010 Apr 28.
We evaluated the activities of mitochondrial respiratory chain complexes in the brain of rats after renal ischemia and the effect of administration of the antioxidants N-acetylcysteine (NAC) and deferoxamine (DFX). The rats were divided into the groups: sham (control) or renal ischemia treated with saline, NAC 20 mg/kg, DFX 20 mg/kg or both antioxidants. Complex I activity was inhibited in hippocampus, striatum, prefrontal cortex and cerebral cortex of rats 1 and 6 h after renal ischemia and that the treatment with a combination of NAC and DFX prevented such effect. Complex I activity was not altered in hippocampus, striatum, prefrontal cortex and cerebral cortex of rats 12 h after renal ischemia. Complexes II and III activities were not altered in hippocampus, striatum, prefrontal cortex and cerebral cortex of rats 1, 6 and 12 h after renal ischemia. Complex IV activity was inhibited in hippocampus, striatum, prefrontal cortex and cerebral cortex of rats 1 h after renal ischemia, but the treatment with the combination of NAC and DFX was able to prevent this inhibition. Complex IV activity was not altered in hippocampus, striatum, prefrontal cortex and cerebral cortex of rats 6 and 12 h after renal ischemia. These results suggest that the inhibition of mitochondrial respiratory chain after renal ischemia might play a role in the pathogenesis of uremic encephalopathy.
我们评估了肾缺血后大鼠大脑中线粒体呼吸链复合物的活性以及抗氧化剂 N-乙酰半胱氨酸 (NAC) 和去铁胺 (DFX) 给药的影响。将大鼠分为以下几组：假手术（对照）或用生理盐水、NAC 20mg/kg、DFX 20mg/kg 或两种抗氧化剂处理的肾缺血。肾缺血 1 和 6 小时后，大鼠海马体、纹状体、前额叶皮质和大脑皮质的复合物 I 活性受到抑制，而联合使用 NAC 和 DFX 可预防这种作用。肾缺血 12 小时后，大鼠海马体、纹状体、前额叶皮质和大脑皮质的复合物 I 活性未改变。肾缺血 1、6 和 12 小时后，大鼠海马体、纹状体、前额叶皮质和大脑皮质的复合物 II 和 III 活性未改变。肾缺血 1 小时后，大鼠海马体、纹状体、前额叶皮质和大脑皮质的复合物 IV 活性受到抑制，但联合使用 NAC 和 DFX 可预防这种抑制。肾缺血 6 和 12 小时后，大鼠海马体、纹状体、前额叶皮质和大脑皮质的复合物 IV 活性未改变。这些结果表明，肾缺血后线粒体呼吸链的抑制可能在尿毒症脑病的发病机制中起作用。
