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酶联免疫吸附测定法(ELISA)在阿米巴病诊断中的应用(作者译)

[The enzyme-linked immunosorbent assay (ELISA) in the diagnosis of amoebiasis (author's transl)].

作者信息

Sorice F, Delia S, Vullo V, Ferone U

出版信息

Ann Sclavo. 1977 May-Jun;19(3):484-9.

PMID:204261
Abstract

An enzyme-linked immunosorbent assay (ELISA) technique as an aid to the detection of antibodies to Entamoeba histolytica has been evaluated in four groups of 39 individuals. Results were positive in 15 patients with amoebic dysentery harboring trophozoites of Entamoeba histolytica, in their stools; negative in 6 patients with enteric symptoms whose faeces contained neither trophozoites nor cysts of Entamoeba histolytica, in 8 patients with other parasitic diseases in whom clinical examination was not indicative of infection with Entamoeba histolytica, and in 10 sera from blood-donors. The ELISA has been shown to be a sensitive method of detecting antibodies to entamoeba histolytica.

摘要

酶联免疫吸附测定(ELISA)技术作为一种辅助检测溶组织内阿米巴抗体的方法,已在四组每组39人的人群中进行了评估。结果显示,15例粪便中带有溶组织内阿米巴滋养体的阿米巴痢疾患者检测结果呈阳性;6例有肠道症状但粪便中既无溶组织内阿米巴滋养体也无包囊的患者检测结果呈阴性;8例临床检查未提示感染溶组织内阿米巴的其他寄生虫病患者检测结果呈阴性;10例献血者血清检测结果也呈阴性。ELISA已被证明是检测溶组织内阿米巴抗体的一种灵敏方法。

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