Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
Unit of Human Biology and Genetics, Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
J Gen Virol. 2010 Aug;91(Pt 8):2054-2061. doi: 10.1099/vir.0.021105-0. Epub 2010 Apr 28.
Epstein-Barr virus (EBV) is ubiquitous in the human population and seroepidemiological studies have revealed that more than 90% of adults are infected with the virus in Thailand. It has been suggested that latent membrane protein 1 (LMP1) variants may differ in their tumorigenicity and geographical localization. The distribution of LMP1 variants of EBV in the Thai population was studied. A total of 259 LMP1 sequences from ten Thai ethnic groups (Lahu, Lisu, Shan, Red Karen, White Karen, Hmong, Akha, Mlabri, Moken and Urak Lawoi) were studied using direct PCR sequencing. Nucleotide sequences corresponding to the C terminus of the LMP1, including previously published sequences from central and southern Thais, were used in the phylogenetic analysis. Five strains--the B95-8 prototype, China 1, China 2, Mediterranean (Med) and SEA 2--were identified in ethnic groups in Thailand. The major strain and the distribution pattern differed by group and location. When the ethnic groups were classified by linguistic group, the prevalence of the SEA 2 strain was significantly different between Austro-Thais and other linguistic groups (P=0.0001), whereas, among Tibeto-Burman linguistic groups, the prevalence of the Med strain was different between matrilocal and patrilocal groups (P=0.0245). The distribution of LMP1 strains in ethnic minorities in Thailand is associated with ethnogeographical factors and the social/marriage system. This study thus provides evidence for the importance of interactions between populations in virus diversity.
EB 病毒（EBV）在人类中普遍存在，血清流行病学研究表明，泰国超过 90%的成年人感染了该病毒。有研究表明，潜伏膜蛋白 1（LMP1）变体在肿瘤发生和地理定位方面可能存在差异。本研究旨在探讨 EBV 在泰国人群中的 LMP1 变体分布。对来自泰国 10 个民族（拉祜族、傈僳族、掸族、红色克伦族、白色克伦族、苗族、阿卡族、Mlabri 族、Moken 族和 Urak Lawoi 族）的 259 个 LMP1 序列进行了直接 PCR 测序研究。使用包括来自泰国中部和南部的已发表序列在内的 LMP1 C 末端核苷酸序列进行了系统发育分析。在泰国各民族中鉴定出了 5 种株系——B95-8 原型株、中国 1 株、中国 2 株、地中海（Med）株和 SEA 2 株。主要株系和分布模式因群体和地理位置而异。当按语言群体对民族进行分类时，SEA 2 株在澳泰语族和其他语言群体之间的流行率存在显著差异（P=0.0001），而在藏缅语族中，Med 株在母系社会和父系社会群体之间的流行率存在差异（P=0.0245）。泰国少数民族中 LMP1 株系的分布与民族地理因素和社会/婚姻制度有关。因此，本研究为人群之间的相互作用对病毒多样性的重要性提供了证据。
