核苷二磷酸酶在与大鼠乳腺高尔基体中乳糖合成相关的尿苷核苷酸循环中的作用。
The role of nucleoside diphosphatase in a uridine nucleotide cycle associated with lactose synthesis in rat mammary-gland Golgi apparatus.
作者信息
Kuhn N J, White A
出版信息
Biochem J. 1977 Dec 15;168(3):423-33. doi: 10.1042/bj1680423.
Abstract
- UDP-galactose utilization by isolated Golgi vesicles or rat mammary gland synthesizing lactose causes accumulation of UMP but not UDP, although UDP is the immediate product of lactose synthase (EC 2.4.1.22). 2. This can be ascribed to a nucleoside diphosphatase (EC 3.6.1.6), specific for UDP, GDP and IDP, activated by bivalent metal ions and apparently located on the luminal face of the Golgi membrane. 3. The uridine diphosphatase activity exceeds the total galactosyltransferase activity 5-fold, and is estimated to maintain UDP at about 14 micrometer within the Golgi lumen. 4. Evidence is given that UMP, but not UDP, penetrates the membrane and that UMP is rephosphorylated to UDP by a UMP kinase located in the cytosol. 5. Golgi-cytosol relationships with respect to lactose synthesis are formulated in terms of a uridine nucleotide cycle which throws new light on the energy cost and possible regulation of lactose synthesis.
摘要
- 分离的高尔基体囊泡或正在合成乳糖的大鼠乳腺利用UDP-半乳糖会导致UMP积累，但不会导致UDP积累，尽管UDP是乳糖合酶（EC 2.4.1.22）的直接产物。2. 这可归因于一种核苷二磷酸酶（EC 3.6.1.6），它对UDP、GDP和IDP具有特异性，由二价金属离子激活，显然位于高尔基体膜的腔面。3. 尿苷二磷酸酶活性超过总半乳糖基转移酶活性5倍，据估计它能使高尔基体腔中的UDP维持在约14微摩尔。4. 有证据表明，UMP而非UDP能穿透膜，且UMP会被位于胞质溶胶中的UMP激酶重新磷酸化为UDP。5. 关于乳糖合成的高尔基体-胞质溶胶关系是根据尿苷核苷酸循环来阐述的，这为乳糖合成的能量消耗和可能的调控提供了新的线索。
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