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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1975 Apr;148(1):77-84. doi: 10.1042/bj1480077.

At short incubation times, and under suitable osmotic conditions, the lactose synthesized by Golgi-derived vesicles of rat mammary gland is 85-90% particulate. Evidence is presented for its occlusion within the lumen of the vesicles. 2. Ovalbumin is used as a bulky active-site inhibitor to show that the active site of lactose synthase lies on the inner face of the Golgi membrane. 3. Phlorrhizin and phloretin inhibit lactose synthesis by such vesicles, indicating the presence of a glucose-transport system. 4. The relationship of this topography to the synthesis of N-acetylneuraminyl-lactose and to the secretion of milk sugars is discussed.

在短时间孵育且处于合适的渗透条件下，大鼠乳腺高尔基体衍生小泡合成的乳糖85 - 90%是颗粒状的。有证据表明其被封闭在小泡腔内。2. 卵清蛋白被用作一种大分子活性位点抑制剂，以表明乳糖合酶的活性位点位于高尔基体膜的内表面。3. 根皮苷和根皮素抑制此类小泡的乳糖合成，表明存在葡萄糖转运系统。4. 讨论了这种拓扑结构与N - 乙酰神经氨酰乳糖合成以及乳中糖类分泌的关系。

Biochem J. 1975 Apr;148(1):77-84. doi: 10.1042/bj1480077.

At short incubation times, and under suitable osmotic conditions, the lactose synthesized by Golgi-derived vesicles of rat mammary gland is 85-90% particulate. Evidence is presented for its occlusion within the lumen of the vesicles. 2. Ovalbumin is used as a bulky active-site inhibitor to show that the active site of lactose synthase lies on the inner face of the Golgi membrane. 3. Phlorrhizin and phloretin inhibit lactose synthesis by such vesicles, indicating the presence of a glucose-transport system. 4. The relationship of this topography to the synthesis of N-acetylneuraminyl-lactose and to the secretion of milk sugars is discussed.

在短时间孵育且处于合适的渗透条件下，大鼠乳腺高尔基体衍生小泡合成的乳糖85 - 90%是颗粒状的。有证据表明其被封闭在小泡腔内。2. 卵清蛋白被用作一种大分子活性位点抑制剂，以表明乳糖合酶的活性位点位于高尔基体膜的内表面。3. 根皮苷和根皮素抑制此类小泡的乳糖合成，表明存在葡萄糖转运系统。4. 讨论了这种拓扑结构与N - 乙酰神经氨酰乳糖合成以及乳中糖类分泌的关系。