乳糖合成的拓扑结构。
The topography of lactose synthesis.
作者信息
Kuhn N J, White A
出版信息
Biochem J. 1975 Apr;148(1):77-84. doi: 10.1042/bj1480077.
Abstract
- At short incubation times, and under suitable osmotic conditions, the lactose synthesized by Golgi-derived vesicles of rat mammary gland is 85-90% particulate. Evidence is presented for its occlusion within the lumen of the vesicles. 2. Ovalbumin is used as a bulky active-site inhibitor to show that the active site of lactose synthase lies on the inner face of the Golgi membrane. 3. Phlorrhizin and phloretin inhibit lactose synthesis by such vesicles, indicating the presence of a glucose-transport system. 4. The relationship of this topography to the synthesis of N-acetylneuraminyl-lactose and to the secretion of milk sugars is discussed.
摘要
- 在短时间孵育且处于合适的渗透条件下，大鼠乳腺高尔基体衍生小泡合成的乳糖85 - 90%是颗粒状的。有证据表明其被封闭在小泡腔内。2. 卵清蛋白被用作一种大分子活性位点抑制剂，以表明乳糖合酶的活性位点位于高尔基体膜的内表面。3. 根皮苷和根皮素抑制此类小泡的乳糖合成，表明存在葡萄糖转运系统。4. 讨论了这种拓扑结构与N - 乙酰神经氨酰乳糖合成以及乳中糖类分泌的关系。
相似文献
1
The topography of lactose synthesis.乳糖合成的拓扑结构。
Biochem J. 1975 Apr;148(1):77-84. doi: 10.1042/bj1480077.
2
GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells.GLUT1 和 GLUT8 支持小鼠乳腺上皮细胞高尔基体中的乳糖合成。
J Physiol Biochem. 2019 Jun;75(2):209-215. doi: 10.1007/s13105-019-00679-3. Epub 2019 Apr 24.
3
Evidence for specific transport of uridine diphosphate galactose across the Golgi membrane of rat mammary gland.尿苷二磷酸半乳糖跨大鼠乳腺高尔基体膜进行特异性转运的证据。
Biochem J. 1976 Jan 15;154(1):243-4. doi: 10.1042/bj1540243.
4
Permeability of lactating-rat mammary gland Golgi membranes to monosaccharides.泌乳大鼠乳腺高尔基体膜对单糖的通透性
Biochem J. 1980 Sep 15;190(3):621-4. doi: 10.1042/bj1900621.
5
Lactose synthesis: the possibilities of regulation.乳糖合成：调控的可能性
J Dairy Sci. 1980 Feb;63(2):328-36. doi: 10.3168/jds.S0022-0302(80)82934-1.
6
The effects of cytochalasin B on glucose transport and lactose synthesis in lactating mammary gland slices.细胞松弛素B对泌乳期乳腺切片中葡萄糖转运和乳糖合成的影响。
Eur J Cell Biol. 1979 Dec;20(2):150-5.
7
The role of nucleoside diphosphatase in a uridine nucleotide cycle associated with lactose synthesis in rat mammary-gland Golgi apparatus.核苷二磷酸酶在与大鼠乳腺高尔基体中乳糖合成相关的尿苷核苷酸循环中的作用。
Biochem J. 1977 Dec 15;168(3):423-33. doi: 10.1042/bj1680423.
8
Biosynthesis of galactinol by lactose synthetase.乳糖合成酶催化棉子糖的生物合成。
Int J Biochem. 1982;14(6):449-51. doi: 10.1016/0020-711x(82)90111-2.
9
-Lactalbumin and galactosyltransferase in rat serum and their relationship to milk secretion.大鼠血清中的乳白蛋白和半乳糖基转移酶及其与乳汁分泌的关系。
Biochem Biophys Res Commun. 1972 Dec 18;49(6):1624-30. doi: 10.1016/0006-291x(72)90528-1.
10
The topography of lactose synthesis. 1975.乳糖合成的拓扑学。1975年。
J Mammary Gland Biol Neoplasia. 2009 Sep;14(3):213-20. doi: 10.1007/s10911-009-9136-6. Epub 2009 Aug 4.
引用本文的文献
1
A Comparative Review of the Cell Biology, Biochemistry, and Genetics of Lactose Synthesis.乳糖合成的细胞生物学、生物化学和遗传学比较综述。
J Mammary Gland Biol Neoplasia. 2021 Jun;26(2):181-196. doi: 10.1007/s10911-021-09490-7. Epub 2021 Jun 14.
2
Biology of glucose transport in the mammary gland.乳腺中葡萄糖转运的生物学。
J Mammary Gland Biol Neoplasia. 2014 Mar;19(1):3-17. doi: 10.1007/s10911-013-9310-8. Epub 2013 Nov 13.
3
Gene regulation of UDP-galactose synthesis and transport: potential rate-limiting processes in initiation of milk production in humans.UDP-半乳糖合成和转运的基因调控：人类泌乳启动的潜在限速过程。
Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E365-76. doi: 10.1152/ajpendo.00175.2012. Epub 2012 May 29.
4
Classic studies of mammary development and milk secretion: 1945 - 1980.乳腺发育与乳汁分泌的经典研究：1945 - 1980年
J Mammary Gland Biol Neoplasia. 2009 Sep;14(3):193-7. doi: 10.1007/s10911-009-9151-7.
5
Introduction: alpha-lactalbumin, a multifunctional protein that specifies lactose synthesis in the Golgi.简介：α-乳白蛋白，一种在高尔基体中参与乳糖合成的多功能蛋白质。
J Mammary Gland Biol Neoplasia. 2009 Sep;14(3):211-2. doi: 10.1007/s10911-009-9149-1.
6
Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35).核苷酸糖转运蛋白家族（SLC35）的分子生理学与病理学
Pflugers Arch. 2004 Feb;447(5):768-75. doi: 10.1007/s00424-003-1093-0. Epub 2003 May 21.
7
Autocrine regulation of milk secretion by a protein in milk.乳汁中一种蛋白质对乳汁分泌的自分泌调节。
Biochem J. 1995 Jan 1;305 ( Pt 1)(Pt 1):51-8. doi: 10.1042/bj3050051.
8
Permeability of lactating-rat mammary gland Golgi membranes to monosaccharides.泌乳大鼠乳腺高尔基体膜对单糖的通透性
Biochem J. 1980 Sep 15;190(3):621-4. doi: 10.1042/bj1900621.
9
Composition, stability and electrolyte permeability of Golgi membranes from lactating-rat mammary gland.哺乳期大鼠乳腺高尔基体膜的组成、稳定性和电解质通透性
Biochem J. 1981 Dec 15;200(3):663-9. doi: 10.1042/bj2000663.
10
Alpha-Lactalbumin and lactose concentrations in rat milk during lactation.哺乳期大鼠乳汁中α-乳白蛋白和乳糖的浓度。
Biochem J. 1981 Jan 15;194(1):149-54. doi: 10.1042/bj1940149.
本文引用的文献
1
GLYCOPEPTIDES FROM OVALBUMIN. PREPARATION, PROPERTIES, AND PARTIAL HYDROLYSIS OF THE ASPARAGINYL CARBOHYDRATE.来自卵清蛋白的糖肽。天冬酰胺基碳水化合物的制备、性质及部分水解
Biochemistry. 1965 Mar;4:566-77. doi: 10.1021/bi00879a030.
2
Synthesis and transfer of amylase in pigeon pancreatic micromosomes.淀粉酶在鸽胰腺微粒体中的合成与转运
J Biol Chem. 1966 Mar 10;241(5):1150-8.
3
The subcellular distribution of the A and B proteins of lactose synthetase in bovine and rat mammary tissue.乳糖合成酶A和B蛋白在牛和大鼠乳腺组织中的亚细胞分布。
J Biol Chem. 1966 Dec 10;241(23):5526-32.
4
Progesterone withdrawal as the lactogenic trigger in the rat.孕酮撤退作为大鼠泌乳的触发因素
J Endocrinol. 1969 May;44(1):39-54. doi: 10.1677/joe.0.0440039.
5
Secretion of alpha-lactalbumin into milk and its relevance to the organization and control of lactose synthetase.α-乳白蛋白向乳汁中的分泌及其与乳糖合成酶的组装和调控的相关性。
Nature. 1969 May 17;222(5194):671-2. doi: 10.1038/222671a0.
6
What regulates lactose content in milk?是什么调节牛奶中的乳糖含量？
Nature. 1969 Mar 8;221(5184):912-4. doi: 10.1038/221912a0.
7
A morphometric study of sub-cellular organization in mouse mammary cancers and normal lactating tissue.小鼠乳腺癌与正常泌乳组织亚细胞结构的形态计量学研究。
Z Zellforsch Mikrosk Anat. 1968;87(2):266-77. doi: 10.1007/BF00319724.
8
Lactogenesis in the rat. Metabolism of uridine diphosphate galactose by mammary gland.大鼠的泌乳发生。乳腺中尿苷二磷酸半乳糖的代谢。
Biochem J. 1968 Feb;106(3):743-8. doi: 10.1042/bj1060743.
9
Galactosyltransferase acceptor specificity of the lactose synthetase A protein.乳糖合成酶A蛋白的半乳糖基转移酶受体特异性
J Biol Chem. 1970 Oct 10;245(19):5057-61.
10
The synthesis of neuramin lactose by preparations of rat mammary gland and its relation to the synthesis of lactose.大鼠乳腺制剂中神经氨酸乳糖的合成及其与乳糖合成的关系。
J Biol Chem. 1969 Nov 25;244(22):6233-40.
Zotero 插件