Medical Microbiology Subdepartment, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
S Afr Med J. 2010 Jan;100(1):53-7.
Helicobacter pylori infection in Kenya is staggeringly high. Evidence links infection of the gastric mucosa by H. pylori with subsequent development of gastric pathologies.
We investigated the prevalence of H. pylori in dyspeptic patients, its relationship with gastric pathologies, and associated antibiotic susceptibility profiles, and compared two media to find the appropriate medium that enhances growth and expedites culture and isolation.
Rapid urease and histological tests were used to screen for H. pylori. Culture was performed to test sensitivity and evaluate media. Selective and nutritional supplements were added to culture media (Colombia blood agar and brain-heart infusion agar) for growth enhancement. E-test strips for metronidazole, amoxicillin and clarithromycin were used for susceptibility testing.
The prevalence of H. pylori infection in children was 73.3%, and 54.8% in adults. All the H. pylori investigated in this study were largely sensitive to clarithromycin (100%, minimum inhibiting concentration (MIC) <2 microg/ml), amoxicillin (100%, MIC <2 microg/ml) and metronidazole (95.4%, MIC <8 microg/ml). There was, however, occasional resistance to metronidazole (4.6%, MIC >8 microg/ml). Both Colombia blood and brain-heart infusion agar, with the supplements, effectively supported H. pylori growth. Growth was achieved in an average of 36 hours for primary isolations and 24 hours for subcultures.
The media described here reduce the time required to culture and isolate bacteria and perform susceptibility testing. Despite the high prevalence of H. pylori infection, the associated pathology is low and does not parallel H. pylori prevalence in the population.
肯尼亚的幽门螺杆菌感染率高得惊人。有证据表明，胃黏膜感染幽门螺杆菌后会随后发展为胃部病变。
我们调查了消化不良患者中幽门螺杆菌的流行情况、其与胃部病变的关系以及相关抗生素药敏谱，并比较了两种培养基，以找到合适的培养基来增强生长并加快培养和分离。
使用快速尿素酶和组织学检测来筛查幽门螺杆菌。进行培养以测试敏感性并评估培养基。向培养基（哥伦比亚血琼脂和脑心浸液琼脂）中添加选择性和营养补充剂以增强生长。使用用于检测甲硝唑、阿莫西林和克拉霉素的 E 试验条进行药敏试验。
儿童幽门螺杆菌感染率为 73.3%，成人感染率为 54.8%。本研究中所有调查的幽门螺杆菌对克拉霉素（100%，最小抑制浓度（MIC）<2μg/ml）、阿莫西林（100%，MIC<2μg/ml）和甲硝唑（95.4%，MIC<8μg/ml）均具有较高的敏感性。然而，甲硝唑偶尔会出现耐药（4.6%，MIC>8μg/ml）。哥伦比亚血琼脂和脑心浸液琼脂添加补充剂后均能有效支持幽门螺杆菌的生长。原发性分离的平均生长时间为 36 小时，传代培养的平均生长时间为 24 小时。
这里描述的培养基可减少培养和分离细菌以及进行药敏试验所需的时间。尽管幽门螺杆菌感染率很高，但相关的病理变化较低，与人群中的幽门螺杆菌流行率并不一致。
