通过酶联免疫吸附测定法检测C型和D型肉毒梭菌毒素

Detection of Clostridium botulinum types C and D toxin by ELISA.

作者信息

Thomas R J

机构信息

Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly.

出版信息

Aust Vet J. 1991 Mar;68(3):111-3. doi: 10.1111/j.1751-0813.1991.tb00769.x.

DOI:10.1111/j.1751-0813.1991.tb00769.x

PMID:2043084

Abstract

An ELISA to detect Clostridium botulinum type D toxin was developed using polyclonal antibodies to a semi-purified toxic complex of the neurotoxin. Sensitivity of the ELISA for detecting type C and type D toxin compared with mouse inoculation was 70% and specificity 96% on samples from animals with botulism diagnosed on clinical signs and herd history. However, both mouse inoculation and the ELISA failed to detect toxin in many animals with a presumptive diagnosis of botulism. Some cross-reaction was seen with Clostridium novyi type A, but not with other clostridial species. While the ELISA described here cannot replace mouse inoculation for the diagnosis of botulism, it is a useful additional test.

摘要

利用针对神经毒素半纯化毒性复合物的多克隆抗体制备了一种用于检测D型肉毒梭菌毒素的酶联免疫吸附测定（ELISA）。与小鼠接种法相比，该ELISA检测C型和D型毒素的灵敏度在根据临床症状和畜群病史诊断为肉毒中毒的动物样本中为70%，特异性为96%。然而，小鼠接种法和ELISA在许多初步诊断为肉毒中毒的动物中均未能检测到毒素。观察到该方法与A型诺维氏梭菌有一些交叉反应，但与其他梭菌属无交叉反应。虽然此处描述的ELISA不能替代小鼠接种法用于肉毒中毒的诊断，但它是一种有用的辅助检测方法。