Gregory A R, Ellis T M, Jubb T F, Nickels R J, Cousins D V
Department of Agriculture, South Perth, Western Australia.
Aust Vet J. 1996 Feb;73(2):55-61. doi: 10.1111/j.1751-0813.1996.tb09966.x.
The development of specific enzyme-linked immunosorbent assays (ELISA) for antibody to types C and D Clostridium botulinum toxins for investigation of botulism in cattle is described. Partially purified type C and D toxins were used as antigens to develop these ELISAs. Specificity of the ELISAs was evaluated on sera from 333 adult beef and dairy cattle from areas with no history or evidence of botulism in animals or water birds. The test was also evaluated on sera from 41 herds that included herds vaccinated against botulism, confirmed botulism cases and herds from areas where the disease is considered endemic. The ELISAs detected the presence of antibody to botulinum toxins in samples from vaccinated cattle and both convalescent and clinically normal animals from unvaccinated herds with outbreaks of botulism. Antibody was also found in unvaccinated animals from herds in which there had been no diagnosed botulism cases in areas where botulism was considered endemic. Sera from some unvaccinated cattle with high ELISA reactivity was shown to be protective for mice in botulinum toxin neutralisation tests. The use of these tests in investigations of botulism in cattle is discussed.
本文描述了用于检测牛肉毒中毒的C型和D型肉毒杆菌毒素抗体的特异性酶联免疫吸附测定（ELISA）的开发。部分纯化的C型和D型毒素用作抗原以开发这些ELISA。在来自动物或水鸟无肉毒中毒病史或证据地区的333头成年肉牛和奶牛的血清上评估了ELISA的特异性。还对来自41个牛群的血清进行了检测，这些牛群包括接种过肉毒中毒疫苗的牛群、确诊的肉毒中毒病例以及来自该病被视为地方病地区的牛群。ELISA检测到接种疫苗的牛以及未接种疫苗且爆发肉毒中毒的牛群中康复期和临床正常动物的样本中存在肉毒杆菌毒素抗体。在肉毒中毒被视为地方病的地区，未接种疫苗且未诊断出肉毒中毒病例的牛群中的动物也检测到了抗体。在肉毒杆菌毒素中和试验中，一些ELISA反应性高的未接种疫苗的牛的血清对小鼠具有保护作用。本文还讨论了这些检测方法在牛肉毒中毒调查中的应用。
