Tagnon Missihoun D, Simeon Kotchoni O
a Department of Biology , Rutgers University , Camden , NJ , USA.
b Center for Computational and Integrative Biology , Rutgers University , Camden , NJ , USA.
Plant Signal Behav. 2017 Nov 2;12(11):e1387707. doi: 10.1080/15592324.2017.1387707. Epub 2017 Oct 9.
Aldehyde molecules were shown to induce gene expression but because of their toxicity, the cell expresses ALDEHYDE DEHYDROGENASES (ALDH, EC 1.2.1.3) that oxidize them to carboxylic acids. To understand how the aldehydes may be both toxic and gene activators, we expressed the ALDH7B4 gene promoter fused to the β-glucuronidase reporter gene in independent transgenic lines and found that pentanal and trans-2-hexenal activated the promoter whereas trans-2-hexenal induced the ALDH7B4 protein. Paraquat led to higher amounts of malondialdehyde compared to trans-2-hexenal and HO, and only the treatment by Paraquat activated the ALDH7B4 promoter, indicating that a threshold level of aldehydes is required for gene activation. These findings suggest that ALDH activity may also serve to fine-tune gene activation by the aldehydes.
醛分子被证明可诱导基因表达，但由于其毒性，细胞会表达醛脱氢酶（ALDH，EC 1.2.1.3），将醛氧化为羧酸。为了解醛如何既具有毒性又能激活基因，我们在独立的转基因系中表达了与β-葡萄糖醛酸酶报告基因融合的ALDH7B4基因启动子，发现戊醛和反式-2-己烯醛可激活该启动子，而反式-2-己烯醛可诱导ALDH7B4蛋白。与反式-2-己烯醛和过氧化氢相比，百草枯导致更高含量的丙二醛，并且只有百草枯处理可激活ALDH7B4启动子，这表明基因激活需要醛达到阈值水平。这些发现表明，ALDH活性也可能用于微调醛对基因的激活作用。
