垂盆草总黄酮的超声提取及大孔树脂纯化工艺研究
[Study on ultrasonic extraction and macroporous resin purification techniques of total flavones from Sedum lineare].
作者信息
Su Jian-Wei, Jia Zhi-Min, Xie Yang
机构信息
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhong Yao Cai. 2013 Feb;36(2):298-301.
OBJECTIVE
To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin.
METHODS
Optimum ultrasonic extraction conditions (ethanol concentration, solid/liquid, ultrasonic time, working temperature) were obtained through orthogonal design. The adsorption ratio and eluting ratio of total flavones were selected as indexes.
RESULTS
The optimum ultrasonic extraction condition was 80% ethanol, solid/liquid 1:40, ultrasonic 20 min at 30 degrees C. The adsorbed AB-8 macroporous resin column was eluted by 80% ethanol at the eluting velocity of 2 mL/min, the eluting ratio and purity of total flavones was 94.72% and 43.51%, respectively.
CONCLUSION
The method is simple and low cost.
目的
优化大孔树脂法对垂盆草中总黄酮的超声提取条件及纯化工艺。
方法
通过正交设计得出最佳超声提取条件（乙醇浓度、固液比、超声时间、工作温度）。选取总黄酮的吸附率和洗脱率作为指标。
结果
最佳超声提取条件为80%乙醇、固液比1:40、30℃超声20分钟。采用AB-8大孔树脂柱吸附，以80%乙醇按2 mL/min的洗脱速度进行洗脱，总黄酮的洗脱率和纯度分别为94.72%和43.51%。
结论
该方法简便且成本低。
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