Wageningen University, Wageningen, The Netherlands.
Trends Biotechnol. 2010 Jun;28(6):308-16. doi: 10.1016/j.tibtech.2010.03.005. Epub 2010 Apr 29.
Various 'omics' methods have enabled environmental probing at the molecular level and have created an important new paradigm in bioremediation design and management. Ecogenomics - the application of genomics to ecological and environmental sciences - defines phylogenetic and functional biodiversity at the DNA, RNA and protein levels. It capitalizes on this knowledge to elucidate functions and interactions of organisms at the ecosystem level in relation to ecological and evolutionary processes. Effective bioremediation of widespread halo-organic pollutants in anaerobic environments requires knowledge of catabolic potential and in situ dynamics of organohalide-respiring and co-metabolizing microorganisms. Here, we discuss the potential of ecogenomics approaches in developing high-throughput methods for detecting and monitoring organohalide respirers, and for providing improvements to selection, specificity and sensitivity of target biomarkers and their application to evaluate bioremediation strategies.
各种“组学”方法使我们能够在分子水平上进行环境探测，并在生物修复设计和管理方面开创了一个重要的新范例。生态基因组学——将基因组学应用于生态和环境科学——定义了 DNA、RNA 和蛋白质水平上的系统发生和功能多样性。它利用这些知识来阐明与生态和进化过程相关的生态系统水平上的生物体的功能和相互作用。要有效地对厌氧环境中广泛存在的卤代有机污染物进行生物修复，就需要了解代谢潜力以及有机卤化物呼吸和共代谢微生物的原位动力学。在这里，我们讨论了生态基因组学方法在开发用于检测和监测有机卤化物呼吸微生物的高通量方法方面的潜力，并为提高目标生物标志物的选择性、特异性和灵敏度及其应用于评估生物修复策略提供了改进。
