Centro de Ciências do Mar do Algarve, Universidade do Algarve, FCMA, Faro, Portugal.
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Mar;6(1):82-91. doi: 10.1016/j.cbd.2010.03.008. Epub 2010 Apr 2.
One of the main constrains for commercial aquaculture production of white seabream (Diplodus sargus) is the high incidence of skeletal malformations in reared fish. The purpose of this study was to obtain a better understanding of the mechanisms involved in the development of these types of skeletal malformations by comparative proteomic analysis of the vertebral column of normal and deformed fish using 2DE for protein separation and MS for protein identification. We observed a 3.2 and 3.4-fold increase in the expression of two tropomyosin isoforms, one of which (tropomyosin-4) is essential for the motility and polarization cycles of osteoclasts. Furthermore, a 1.6, 1.7 and 1.8-fold increase in three parvalbumin spots was detected, suggesting a cellular response to increased intracellular Ca²(+) levels. These results can be interpreted as signs of increased cellular activity in the bone of white seabream with skeletal deformities coupled to a higher degree of calcium mobilization, which elicits further studies into the use of these proteins as indicators of skeletal metabolic state.
养殖白鲷（Diplodus sargus）骨骼畸形发生率高，是商业水产养殖的主要制约因素之一。本研究通过比较正常鱼和畸形鱼脊柱的二维电泳（2DE）分离蛋白和质谱（MS）鉴定蛋白的蛋白质组学分析，旨在更好地了解这些骨骼畸形的发生机制。我们观察到两种原肌球蛋白同工型的表达分别增加了 3.2 倍和 3.4 倍，其中一种（原肌球蛋白-4）对于破骨细胞的运动和极化周期是必需的。此外，还检测到三个副肌球蛋白斑点的表达分别增加了 1.6 倍、1.7 倍和 1.8 倍，表明细胞对细胞内 Ca²（+）水平升高的反应。这些结果可以解释为骨骼畸形的白鲷骨骼中细胞活性增加的迹象，伴随着钙动员程度的提高，这引发了进一步研究这些蛋白质作为骨骼代谢状态指标的用途。
