CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):757-761. doi: 10.1099/ijs.0.022558-0. Epub 2010 Apr 30.
A Gram-stain-positive, aerobic, non-motile actinomycete strain, designated YIM 65601(T), was studied by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain YIM 65601(T) should be assigned to the genus Nonomuraea, with highest 16S rRNA gene sequence similarities to Nonomuraea candida HMC10(T) (98.8 %), N. salmonea DSM 43678(T) (98.7 %), N. turkmeniaca DSM 43926(T) (98.5 %), N. roseola DSM 43767(T) (98.4 %), N. dietziae IFO 14309(T) (98.2 %) and N. kuesteri GW 14-1925(T) (98.1 %). 16S rRNA gene sequence similarities to strains of other Nonomuraea species were below 98.0 %. Morphological and chemotaxonomic properties of the isolate were consistent with those of members of the genus Nonomuraea. The results of DNA-DNA hybridization and physiological and biochemical tests and fatty acid profiles allowed genotypic and phenotypic differentiation of strain YIM 65601(T) from closely related species. Thus, YIM 65601(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea endophytica sp. nov. is proposed, with YIM 65601(T) ( = CCTCC AA 209037(T) = DSM 45385(T)) as the type strain.
一株革兰氏阳性、需氧、非运动放线菌菌株 YIM 65601(T)，通过多相分类法进行了研究。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 YIM 65601(T)应归属于游动放线菌属，与游动放线菌属 Nonomuraea candida HMC10(T)（98.8%）、N. salmonea DSM 43678(T)（98.7%）、N. turkmeniaca DSM 43926(T)（98.5%）、N. roseola DSM 43767(T)（98.4%）、N. dietziae IFO 14309(T)（98.2%）和 N. kuesteri GW 14-1925(T)（98.1%）的 16S rRNA 基因序列相似度最高。与其他游动放线菌属种的菌株的 16S rRNA 基因序列相似度低于 98.0%。该分离物的形态学和化学分类学特性与游动放线菌属的成员一致。DNA-DNA 杂交和生理生化试验以及脂肪酸图谱的结果允许菌株 YIM 65601(T)从密切相关的种进行基因和表型分化。因此，YIM 65601(T)代表游动放线菌属的一个新种，提议将其命名为内生游动放线菌（Nonomuraea endophytica）sp. nov.，其模式菌株为 YIM 65601(T)（ = CCTCC AA 209037(T) = DSM 45385(T)）。
