Huang Huiqin, Liu Min, Zhong Weida, Mo Kunlian, Zhu Jun, Zou Xiaoxiao, Hu Yonghua, Bao Shixiang
Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3144-3148. doi: 10.1099/ijsem.0.002954. Epub 2018 Sep 4.
A novel aerobic actinomycete, designated HA15826, was isolated from a mangrove soil sample collected in Sanya, China. Scanning electron microscopy revealed that the isolate produced straight to slightly flexural spore chains with rough cylindrical spores. Chemotaxonomic tests showed that the cell wall contained meso-diaminopimelic acid and the major fatty acids were iso-C16 : 0, 10-methyl-C17 : 0, C17 : 1ω8c and C16 : 0. 16S rRNA gene sequence similarity analysis showed that strain HA15826 belonged to the genus Nonomuraea, being most closely related to Nonomuraea dietziae DSM 44320 (98.7 %), Nonomuraea candida HMC10 (98.4 %), Nonomuraea africana IFO 14745 (98.4 %), Nonomuraea roseola IFO 14685 (98.2 %) and Nonomuraea recticatena IFO 14525 (98.1 %). The DNA G+C content of the type strain is 73.2 %. DNA-DNA relatedness and comparative analyses of physiological, biochemical and chemotaxonomic data allowed genotypic and phenotypic differentiation of strain HA15826 from the closely related species. Thus, strain HA15826 should be classified as a novel species of the genus Nonomuraea, for which the name Nonomuraeamangrovi sp. nov. is proposed. The type strain is HA15826 (=CGMCC 4.7425=DSM 105694).
从中国三亚采集的红树林土壤样本中分离出一株新型好氧放线菌，命名为HA15826。扫描电子显微镜显示，该分离株产生直链至微弯曲的孢子链，孢子呈粗糙的圆柱形。化学分类学测试表明，细胞壁含有内消旋二氨基庚二酸，主要脂肪酸为异-C16 : 0、10-甲基-C17 : 0、C17 : 1ω8c和C16 : 0。16S rRNA基因序列相似性分析表明，菌株HA15826属于野野村氏菌属，与迪茨野野村氏菌DSM 44320（98.7 %）、白色野野村氏菌HMC10（98.4 %）、非洲野野村氏菌IFO 14745（98.4 %）、玫瑰野野村氏菌IFO 14685（98.2 %）和直链野野村氏菌IFO 14525（98.1 %）关系最为密切。模式菌株的DNA G+C含量为73.2 %。DNA-DNA相关性以及生理、生化和化学分类学数据的比较分析使得菌株HA15826能够与相近物种在基因型和表型上区分开来。因此，菌株HA15826应被归类为野野村氏菌属的一个新物种，为此提出新名称红树林野野村氏菌（Nonomuraea mangrovi sp. nov.）。模式菌株为HA15826（=CGMCC 4.7425=DSM 105694）。
