College of Resources and Environment, Northwest A & F University, 712100 Yangling, PR China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1587-1591. doi: 10.1099/ijs.0.034751-0. Epub 2011 Sep 2.
A straight-chain, spore-forming actinobacterium, strain YIM 120770(T), was isolated from soil. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons revealed that the isolate represents a distinct cluster within the clade comprising the genus Nonomuraea and is related most closely to Nonomuraea rhizophila YIM 67092(T) (96.5% similarity). Cells of strain YIM 120770(T) grew in the presence of 0-3% (w/v) NaCl, at 15-37 °C and at pH 7.0-8.0. The diagnostic amino acid was meso-diaminopimelic acid, cell hydrolysates contained madurose, glucose, mannose, ribose and galactose, the predominant cellular fatty acids were 10-methyl C(17:0) and iso-C(16:0), and the DNA G+C content was 66.4 mol%, data consistent with affiliation of strain YIM 120770(T) to the genus Nonomuraea. Strain YIM 120770(T) shared low levels of 16S rRNA gene sequence similarity (<97%) with the type strains of recognized species of the genus Nonomuraea and could be differentiated from its closest phylogenetic relative based on phenotypic characteristics. These results suggested that strain YIM 120770(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea soli sp. nov. is proposed. The type strain is YIM 120770(T) (=DSM 45533(T)=JCM 17347(T)).
一株直链、产孢子的放线菌，菌株 YIM 120770(T)，从土壤中分离得到。基于 16S rRNA 基因序列比较的系统发育分析表明，该分离物代表了包含诺氏菌属的分支内的一个独特群，与 Nonomuraea rhizophila YIM 67092(T)（相似度 96.5%）最为密切相关。菌株 YIM 120770(T)的细胞在 0-3%（w/v）NaCl、15-37°C 和 pH 7.0-8.0 的条件下生长。诊断氨基酸为 meso-二氨基庚二酸，细胞水解产物含有马杜糖、葡萄糖、甘露糖、核糖和半乳糖，主要细胞脂肪酸为 10-甲基 C(17:0)和 iso-C(16:0)，DNA G+C 含量为 66.4 mol%，这些数据与菌株 YIM 120770(T)属于诺氏菌属的归属一致。菌株 YIM 120770(T)与公认的诺氏菌属种的模式菌株的 16S rRNA 基因序列相似度（<97%）较低，并且可以基于表型特征与最接近的系统发育相关物区分开来。这些结果表明，菌株 YIM 120770(T)代表了诺氏菌属的一个新种，建议将其命名为 Nonomuraea soli sp. nov.。其模式菌株为 YIM 120770(T)=DSM 45533(T)=JCM 17347(T)。
