Hee H T, Ismail H D, Lim C T, Goh J C H, Wong H K
Department of Orthopaedic Surgery, National University Hospital, Orthopaedic and Hand Reconstruction Surgery Cluster, National University Health System, 5 Lower Kent Ridge Road, Singapore.
J Bone Joint Surg Br. 2010 May;92(5):726-36. doi: 10.1302/0301-620X.92B5.23015.
Although success has been achieved with implantation of bone marrow mesenchymal stem cells (bMSCs) in degenerative discs, its full potential may not be achieved if the harsh environment of the degenerative disc remains. Axial distraction has been shown to increase hydration and nutrition. Combining both therapies may have a synergistic effect in reversing degenerative disc disease. In order to evaluate the effect of bMSC implantation, axial distraction and combination therapy in stimulating regeneration and retarding degeneration in degenerative discs, we first induced disc degeneration by axial loading in a rabbit model. The rabbits in the intervention groups performed better with respect to disc height, morphological grading, histological scoring and average dead cell count. The groups with distraction performed better than those without on all criteria except the average dead cell count. Our findings suggest that bMSC implantation and distraction stimulate regenerative changes in degenerative discs in a rabbit model.
尽管将骨髓间充质干细胞（bMSCs）植入退变椎间盘中已取得成功，但如果退变椎间盘的恶劣环境依然存在，其全部潜能可能无法实现。轴向撑开已被证明可增加水合作用和营养供应。联合应用这两种疗法可能在逆转椎间盘退变疾病方面具有协同效应。为了评估bMSC植入、轴向撑开及联合疗法对刺激退变椎间盘中再生和延缓退变的效果，我们首先在兔模型中通过轴向加载诱导椎间盘退变。干预组的兔子在椎间盘高度、形态学分级、组织学评分及平均死细胞计数方面表现更佳。除平均死细胞计数外，撑开组在所有标准上均比未撑开组表现更好。我们的研究结果表明，bMSC植入和撑开可刺激兔模型退变椎间盘中的再生性变化。
