Dipartimento di Clinica Veterinaria, Università di Pisa, Pisa, Italy.
Vet Res Commun. 2010 Jun;34 Suppl 1:S79-82. doi: 10.1007/s11259-010-9395-0.
Methicillin and multi-drug resistance were investigated in 136 Staphylococcus intermedius strains of canine origin. The large majority of isolates were found to be mecA-negative by polymerase chain reaction, whereas only four strains were positive for the mecA gene. All mecA-positive strains were confirmed as methicillin-resistant by complementary tests, except for oxacillin disk diffusion, which yielded one false-negative result. A significantly higher resistance to fusidic acid, lincosamides, and cotrimoxazole was observed in methicillin-resistant S. intermedius (MRSI) compared with methicillin-susceptible strains. Although the prevalence of MRSI in dogs appeared to be low, methicillin resistance was confirmed to be associated with multi-drug resistance, suggesting the importance of antimicrobial susceptibility testing of canine S. intermedius strains.
我们调查了 136 株来自犬源的中间葡萄球菌，研究了其耐甲氧西林和多药耐药情况。聚合酶链反应结果显示，绝大多数分离株均为 mecA 阴性，而 mecA 基因阳性的菌株仅有 4 株。除了苯唑西林纸片扩散试验有 1 个假阴性结果外，所有 mecA 阳性菌株均通过补充试验确认为耐甲氧西林，除了对万古霉素和利福平之外。耐甲氧西林中间葡萄球菌（MRSI）对夫西地酸、林可酰胺类和复方新诺明的耐药率明显高于甲氧西林敏感株。尽管犬源 MRSI 的流行率似乎较低，但耐甲氧西林与多药耐药有关，这表明对犬源中间葡萄球菌进行抗菌药物敏感性试验很重要。
