Sasaki Asako, Shimizu Akira, Kawano Junichi, Wakita Yoshihisa, Hayashi Toshikatsu, Ootsuki Shigenobu
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Rokkodai-cho, Nada-ku, Kobe-shi, Hyogo, Japan.
J Vet Med Sci. 2005 Jan;67(1):103-6. doi: 10.1292/jvms.67.103.
Staphylococcus intermedius isolates from diseased and healthy dogs were examined for production of extracellular enzymes and toxins, and phage patterns. There were no significant differences between the two groups of isolates in the production rates of DNase, protease, lipase, gelatinase, hyaluronidase, hemolysins, protein A, and TSST-1, or in phage patterns. But the production rate of enterotoxins in isolates from diseased dogs was significantly higher than that in isolates from healthy dogs. PFGE analysis was performed with isolates from different body sites in individual dogs. In 3 of 6 healthy dogs, identical PFGE patterns were seen in isolates from the nares, external auditory meatus or skin. The remaining 3 dogs yielded isolates of different patterns. In 4 of 6 diseased dogs, identical patterns were seen in isolates from lesions as well as from the other normal sites.
对从患病和健康犬只分离出的中间葡萄球菌进行了细胞外酶和毒素产生情况以及噬菌体分型检测。两组分离株在脱氧核糖核酸酶、蛋白酶、脂肪酶、明胶酶、透明质酸酶、溶血素、蛋白A和毒性休克综合征毒素-1的产生率或噬菌体分型方面没有显著差异。但是，患病犬只分离株中肠毒素的产生率显著高于健康犬只分离株。对个别犬只不同身体部位的分离株进行了脉冲场凝胶电泳（PFGE）分析。在6只健康犬中的3只，从鼻孔、外耳道或皮肤分离出的菌株呈现相同的PFGE图谱。其余3只犬只分离出的菌株具有不同的图谱。在6只患病犬中的4只，从病变部位以及其他正常部位分离出的菌株呈现相同的图谱。
