Department of Psychiatry, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Biol Reprod. 2010 Aug 1;83(2):220-7. doi: 10.1095/biolreprod.109.082826. Epub 2010 May 5.
Fortunately, the majority of children conceived through assisted reproductive technologies (ARTs) appear healthy; however, metabolic abnormalities, including elevated glucose and increased and altered adipose tissue deposition, have been reported in adolescents. To parse out factors that may be responsible, we investigated the effects of two different ARTs--in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI)--as well as somatic cell nuclear transfer (SCNT) on glucose clearance, body weight, and body composition of young adult mice. Female and male mice generated through ART weighed more than control (naturally conceived [STOCK]) mice at birth. No differences in body weight were observed in males up to 8 wk of age. ART females took longer than control mice to clear a glucose bolus, with glucose clearance most impaired in SCNT females. IVF females secreted more insulin and had a higher insulin peak 15 min after glucose injection compared with all other groups. Male mice exhibited no differences in glucose clearance, but IVF males required more insulin to do so. SCNT females weighed more than IVF, ICSI, and STOCK females, and they had higher fat content than ICSI females and higher leptin levels than all other groups. These results show that glucose parameters are altered in young adult mice conceived through techniques associated with ART before onset of obesity and may be responsible for its development later in life. The present study suggests that more investigation regarding the long-term effects of manipulations associated with ART is warranted.
幸运的是，大多数通过辅助生殖技术（ART）受孕的儿童看起来都很健康；然而，代谢异常，包括葡萄糖升高和脂肪组织沉积增加和改变，在青少年中已有报道。为了梳理出可能导致这些问题的因素，我们研究了两种不同的辅助生殖技术——体外受精（IVF）和胞浆内精子注射（ICSI）以及体细胞核转移（SCNT）对年轻成年小鼠葡萄糖清除率、体重和体成分的影响。通过 ART 产生的雌性和雄性小鼠在出生时比对照（自然受孕[STOCK]）小鼠重。在 8 周龄之前，雄性小鼠的体重没有差异。ART 雌性小鼠清除葡萄糖冲击所需的时间长于对照小鼠，而 SCNT 雌性小鼠的葡萄糖清除率受损最为严重。IVF 雌性小鼠分泌的胰岛素更多，在葡萄糖注射后 15 分钟时胰岛素峰值更高，与其他所有组相比均如此。雄性小鼠的葡萄糖清除率没有差异，但 IVF 雄性小鼠需要更多的胰岛素来实现。SCNT 雌性小鼠比 IVF、ICSI 和 STOCK 雌性小鼠重，脂肪含量比 ICSI 雌性小鼠高，瘦素水平比其他所有组都高。这些结果表明，在肥胖发生之前，通过与 ART 相关的技术受孕的年轻成年小鼠的葡萄糖参数发生改变，可能是其日后发生肥胖的原因。本研究表明，需要进一步研究与 ART 相关的操作的长期影响。
