Department of Ophthalmology, King Saud University, Riyadh, Saudi Arabia.
Clin Exp Ophthalmol. 2010 Jan;38(1):57-64; quiz 87. doi: 10.1111/j.1442-9071.2010.02194.x.
To investigate associations between expressions of advanced glycation end products (AGEs), transforming growth factor-beta (TGF-beta), tumour necrosis factor-alpha (TNF-alpha) and integrins and correlations between their expression and level of vascularization and proliferative activity in diabetic fibrovascular epiretinal membranes.
Membranes from eight patients with active proliferative diabetic retinopathy and nine patients with inactive proliferative diabetic retinopathy were studied by immunohistochemistry.
Blood vessels expressed AGEs, TGF-beta, TNF-alpha and alpha(v)beta(3) integrin in 5, 13, 8 and 8 membranes, respectively. Stromal cells expressed AGEs, TNF-alpha and alpha(v)beta(3) integrin in 15, 13 and 3 membranes, respectively. There was no immunoreactivity for alpha(v)beta(5), alpha(5)beta(1) and alpha(2)beta(1) integrins. There were significant correlations between number of blood vessels expressing CD34 and number of blood vessels expressing AGEs (r(s) = 0.496; P = 0.043), TGF-beta (r(s) = 0.777; P < 0.001) and TNF-alpha (r(s) = 0.699; P = 0.002). There were significant correlations between number of blood vessels expressing AGEs and number of blood vessels expressing TGF-beta (r(s) = 0.532; P = 0.028) and TNF-alpha (r(s) = 0.626; P = 0.007). The correlation between number of blood vessels expressing TNF-alpha and alpha(v)beta(3) integrin was significant (r(s) = 0.617; P = 0.008). Number of blood vessels expressing CD34 (P = 0.001), TGF-beta (P = 0.006) and TNF-alpha (P = 0.002) and stromal cells expressing AGEs (P = 0.001) and TNF-alpha (P = 0.004) were significantly higher in active membranes than in inactive membranes.
Interactions of AGEs, TGF-beta, TNF-alpha and alpha(v)beta(3) integrin might be involved in pathogenesis of proliferative diabetic retinopathy fibrovascular proliferation.
研究晚期糖基化终产物 (AGEs)、转化生长因子-β (TGF-β)、肿瘤坏死因子-α (TNF-α) 和整合素的表达与糖尿病性纤维血管性视网膜前膜血管化和增殖活性水平之间的关系及其表达之间的关系。
对 8 例活动期增生性糖尿病视网膜病变和 9 例非活动期增生性糖尿病视网膜病变患者的膜进行免疫组织化学研究。
血管在 5、13、8 和 8 个膜中分别表达 AGEs、TGF-β、TNF-α 和 α(v)β(3)整合素。基质细胞在 15、13 和 3 个膜中分别表达 AGEs、TNF-α和 α(v)β(3)整合素。没有α(v)β(5)、α(5)β(1)和 α(2)β(1)整合素的免疫反应。CD34 阳性血管数与 AGEs(r(s)=0.496；P=0.043)、TGF-β(r(s)=0.777；P<0.001)和 TNF-α(r(s)=0.699；P=0.002)表达的血管数呈显著相关。AGEs 表达的血管数与 TGF-β(r(s)=0.532；P=0.028)和 TNF-α(r(s)=0.626；P=0.007)表达的血管数呈显著相关。TNF-α与 α(v)β(3)整合素的表达呈显著相关(r(s)=0.617；P=0.008)。CD34 阳性血管数(P=0.001)、TGF-β(P=0.006)和 TNF-α(P=0.002)以及基质细胞表达 AGEs(P=0.001)和 TNF-α(P=0.004)在活动期膜中明显高于非活动期膜。
AGEs、TGF-β、TNF-α 和 α(v)β(3)整合素的相互作用可能参与增生性糖尿病性视网膜病变纤维血管增殖的发病机制。
