El-Asrar A M Abu, Missotten L, Geboes K
Department of Ophthalmology, King Abdulaziz University Hospital, Old Airport Road, Riyadh, Saudi Arabia.
Br J Ophthalmol. 2008 Nov;92(11):1534-9. doi: 10.1136/bjo.2008.142182.
BACKGROUND/AIMS: The inducible enzyme cyclo-oxygense-2 (COX-2) and its metabolic products are important mediators for angiogenesis. We investigated the expression of COX-2 and its downstream enzymes microsomal prostaglandin-E synthase (mPGES)-1, cytosolic PGES (cPGES) and thromboxane synthase (TXS), and correlated it with vascular endothelial growth factor (VEGF) expression and level of vascularisation in proliferative diabetic retinopathy (PDR) epiretinal membranes.
Membranes from five patients with active PDR and nine patients with inactive PDR were studied by immunohistochemistry.
Vascular endothelial cells expressed COX-2, mPGES-1 and VEGF in 11, nine and six membranes, respectively. TXS was expressed in stromal cells in 12 membranes. There was no immunoreactivity for cPGES. There were significant correlations between number of blood vessels expressing CD34 and number of blood vessels expressing COX-2 (r(s) = 0.858; p<0.001), mPGES-1 (r(s) = 0.743; p = 0.002) and VEGF (r(s) = 0.845; p = 0.001) and number of cells expressing TXS (r(s) = 0.74; p = 0.002). The number of blood vessels expressing CD34 (p = 0.007), COX-2 (p = 0.027) and VEGF (p = 0.008) and stromal cells expressing mPGES-1 (p = 0.003), TXS (p = 0.04) and VEGF (p = 0.017) were significantly higher in active membranes than in inactive membranes.
COX-2 and its metabolic products might contribute to PDR angiogenesis.
背景/目的：诱导型酶环氧化酶-2（COX-2）及其代谢产物是血管生成的重要介质。我们研究了COX-2及其下游酶微粒体前列腺素-E合酶（mPGES）-1、胞质PGES（cPGES）和血栓素合酶（TXS）的表达，并将其与增殖性糖尿病视网膜病变（PDR）视网膜前膜中的血管内皮生长因子（VEGF）表达及血管化程度相关联。
采用免疫组织化学方法研究了5例活动性PDR患者和9例非活动性PDR患者的视网膜前膜。
血管内皮细胞分别在11、9和6个视网膜前膜中表达COX-2、mPGES-1和VEGF。TXS在12个视网膜前膜的基质细胞中表达。cPGES无免疫反应性。表达CD34的血管数量与表达COX-2（rs = 0.858；p<0.001）、mPGES-1（rs = 0.743；p = 0.002）和VEGF（rs = 0.845；p = 0.001）的血管数量以及表达TXS的细胞数量（rs = 0.74；p = 0.002）之间存在显著相关性。活动性视网膜前膜中表达CD34（p = 0.007）、COX-2（p = 0.027）和VEGF（p = 0.008）的血管数量以及表达mPGES-1（p = 0.003）、TXS（p = 0.04）和VEGF（p = 0.017）的基质细胞数量显著高于非活动性视网膜前膜。
COX-2及其代谢产物可能有助于PDR的血管生成。
