Unidade de Helmintologia e Malacologia Médicas/UPMM, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal.
Exp Parasitol. 2010 Oct;126(2):191-7. doi: 10.1016/j.exppara.2010.04.023. Epub 2010 May 4.
The anthelmintic activity of extracts from Chenopodiumambrosioides, Pycnanthusangolensis and Nutridesintox was in vitro and in vivo investigated, against Toxocaracanis larvae. The in vitro assays results showed that the aqueous extract of Nutridesintox was the most effective, followed by C. ambrosioides extracts, hexane, dichloromethane and the infusion. P. angolensis extracts showed a lower anthelmintic activity compared to the other natural products. For the in vivo assays, Nutridesintox, the hexane extract and the infusion of C. ambrosioides were administered orally to T. canis-infected mice, in single doses, during three consecutive days. The efficacy was evaluated on the 17th day post-infection, not only by counting T. canis larvae in the tissues but also by ELISA detection of IgM and IgG antibodies and histological analysis of liver and lungs. The different treatments did not reduce the larvae burden and had no influence on the antibodies dynamic. Interestingly, a reduction on the inflammatory infiltrates was observed in the liver and lung sections of the group treated with the hexane extract of C. ambrosioides. In conclusion, the hexane extract of C. ambrosioides is of further research interest, as it showed an anthelmintic activity in vitro and a reduction on the inflammatory reaction produced by the infection of T. canis larvae in vivo.
研究了藜、安哥拉山龙眼和 Nutridesintox 的提取物对犬弓首蛔虫幼虫的体内外驱虫活性。体外试验结果表明，Nutridesintox 的水提物最有效，其次是藜提取物、正己烷、二氯甲烷和浸液。与其他天然产物相比，安哥拉山龙眼提取物的驱虫活性较低。对于体内试验，在连续三天内，单次口服给予犬弓首蛔虫感染小鼠 Nutridesintox、正己烷提取物和藜浸液。在感染后第 17 天，不仅通过计数组织中的犬弓首蛔虫幼虫，还通过 ELISA 检测 IgM 和 IgG 抗体以及肝脏和肺组织的组织学分析来评估疗效。不同的治疗方法并没有减轻幼虫负担，也没有影响抗体的动态。有趣的是，用藜正己烷提取物处理的组的肝脏和肺组织切片中观察到炎症浸润减少。总之，藜的正己烷提取物具有进一步研究的意义，因为它在体外具有驱虫活性，并且在体内减轻了犬弓首蛔虫幼虫感染引起的炎症反应。
