Akkus Gozde Nur, Atalay Tugrul, Akdeniz Sinem, Ekici Husamettin, Yildiz Dincer, Gurcan I Safa, Yildiz Kader
Health Sciences Institute, Department of Parasitology, Kirikkale University, Kirikkale, 71450, Türkiye.
Sefaatli Vocational School, Yozgat Bozok University, Yozgat, 66800, Türkiye.
Acta Parasitol. 2025 Jan 24;70(1):31. doi: 10.1007/s11686-024-00970-4.
In the present study, the effects of leaf and rhizome extracts of Arum rupicola Boiss rupicola were searched on the infective stage Toxocara canis larvae (L3) in the experimentally infected mice.
Four-six week-old male BALB/c mice were divided into eight groups (G1-8, each group consisted of 7 mice), and they were infected orally with 500 T. canis eggs with L3. After 24 h, the following treatment protocol was applied to the groups for five days: G-1: Albendazole (ABZ) (100 mg/kg), G-2: Leaf Extract (10 mg/mL) + ABZ (100 mg/kg), G-3: Leaf Extract (40 mg/mL) + ABZ (100 mg/kg), G-4: Rhizome Extract + ABZ (100 mg/kg), G-5: Leaf Extract (10 mg/mL), G-6: Leaf Extract (40 mg/mL), G-7: Rhizome extract, G-8: Negative control. The necropsy was performed on the 45th day of experimental infection.
The rhizome extract significantly reduced the effectiveness of ABZ compared to leaf extracts. The number of larvae in rhizome extract-treated mice (G7) was similar to ABZ-treated mice (G1) (P < 0.05). The larval number in mice administered leaf extract (10 mg/mL and 40 mg/mL) was slightly decreased. But no significant difference was detected in the larval number depending on the dose of leaf extracts (P > 0.05).
The administration of the leaf and rhizome extracts did not contribute to the effectiveness of ABZ. Also the effect of ABZ on the larvae number obtained from the tissues was reduced by the rhizome extract. The larvae number in the group in which rhizome extract was given alone was almost close to the group in which ABZ was administered. The results provide insight for further research on the antiparasitic properties of A. rupicola Boiss rupicola rhizome extract.
在本研究中，探究了岩芋叶提取物和根茎提取物对实验感染小鼠体内犬弓首蛔虫感染期幼虫（L3）的影响。
将4至6周龄的雄性BALB/c小鼠分为八组（G1 - 8，每组7只小鼠），经口感染500枚含L3的犬弓首蛔虫卵。24小时后，对各实验组进行为期五天的如下处理：G - 1：阿苯达唑（ABZ）（100毫克/千克）；G - 2：叶提取物（10毫克/毫升） + ABZ（100毫克/千克）；G - 3：叶提取物（40毫克/毫升） + ABZ（100毫克/千克）；G - 4：根茎提取物 + ABZ（100毫克/千克）；G - 5：叶提取物（10毫克/毫升）；G - 6：叶提取物（40毫克/毫升）；G - 7：根茎提取物；G - 8：阴性对照。在实验感染的第45天进行剖检。
与叶提取物相比，根茎提取物显著降低了ABZ的疗效。接受根茎提取物处理的小鼠（G7）体内幼虫数量与接受ABZ处理的小鼠（G1）相似（P < 0.05）。给予叶提取物（10毫克/毫升和40毫克/毫升）的小鼠体内幼虫数量略有减少。但根据叶提取物剂量的不同，幼虫数量未检测到显著差异（P > 0.05）。
叶提取物和根茎提取物的给药并未提高ABZ的疗效。此外，根茎提取物降低了ABZ对从组织中获取的幼虫数量的影响。单独给予根茎提取物组的幼虫数量几乎与给予ABZ组相近。这些结果为进一步研究岩芋根茎提取物的抗寄生虫特性提供了思路。
